黄金万红膏对SD大鼠深Ⅱ度烫伤创面修复作用及机制研究  

Study on the effect and mechanism of Huangjinwanhong ointment on deepⅡdegree scald wound in SD rats

作　　者：徐莉霞 蔡政 徐永清 刘磊 何蔚楹 钟晓君 XU Lixia;CAI Zheng;XU Yongqing;LIU Lei;HE Weiying;ZHONG Xiaojun(Department of Nursing,Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650500,China/First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650021,China;Department of Oncology,Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650500,China/First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650021,China;Department of Anesthesiology,Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650500,China/First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650021,China;Medical Records Statistics Section,Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650500,China/First Affiliated Hospital of Yunnan University of Traditional Chinese Medicine,Kunming Yunnan 650021,China;Department of orthopaedics,920th Hospital of Joint Logistic Support Force of PLA,Kunming Yunnan 650500,China;Department of surgery,Songjiang District Hospital of Traditional Chinese Medicine of Shanghai,Shanghai 201600,China)

机构地区：[1]云南省中医医院/云南中医药大学第一附属医院护理部,云南昆明650021 [2]云南省中医医院/云南中医药大学第一附属医院肿瘤科,云南昆明650021 [3]云南省中医医院/云南中医药大学第一附属医院麻醉科,云南昆明650021 [4]云南省中医医院/云南中医药大学第一附属医院病案统计科,云南昆明650021 [5]中国人民解放军联勤保障部队第九二〇医院骨科,云南昆明650500 [6]上海市松江区方塔中医医院外科,上海201600

出　　处：《云南医药》2025年第1期80-83,共4页Medicine and Pharmacy of Yunnan

基　　金：云南中医药大学院校联合基金项目(YXLH202111)。

摘　　要：目的探讨黄金万红膏对SD大鼠深Ⅱ度烫伤创面愈合的影响及其可能的作用机制。方法予黄金万红膏干预深Ⅱ度烫伤的建立SD大鼠,观察各组SD大鼠创面愈合情况,检测受损组织中Wnt信号通路的Wnt1、β-catenin mRNA及蛋白的表达。结果与模型组相比,实验组创口面积、愈合时间差异具有统计学意义(P<0.01)。与模型组相比,实验组烫伤部位皮肤真皮层恢复较多,炎症细胞浸润减少。与模型组相比,实验组中Wnt 1 mRNA水平表达差异具有统计学意义(P<0.05)。相比模型组,实验组Wnt 1、β-catenin的蛋白表达差异具有统计学意义(P<0.05)。结论黄金万红膏可能通过调控Wnt信号通路,调节Wnt 1、β-catenin相关基因及蛋白的表达,起到促进深Ⅱ度烫伤创面修复的作用。Objective To investigate the effect of Huangjinwanhong ointment on wound healing of deepⅡdegree scald in SD rats and its possible mechanism.Methods SD rats were treated with Huangjinwanhong ointment,and the wound healing of SD rats was observed.The expression of Wnt1 andβ-catenin mRNA and protein of Wnt signaling pathway in the damaged tissues were detected.Results Compared with model group,the difference of wound area and healing time in experimental group was statistically significant(P<0.01).Compared with the model group,the dermis of the burn site in the experimental group recovered more and the inflammatory cell infiltration decreased.Compared with the model group,the mRNA level of Wnt 1 in the experimental group was significantly different(P<0.05).Compared with the model group,the protein expressions of WNT-1 andβ-catenin in the experimental group were significantly different(P<0.05).Conclusions Huangjinwanhong ointment may regulate the expression of Wnt 1 andβ-catenin related genes and proteins by regulating the Wnt signaling pathway,and play a role in promoting the wound repair of deepⅡdegree scald.

关 键 词：黄金万红膏 深Ⅱ度烫伤 创面修复 SD大鼠 WNT信号通路 

分 类 号：R285.5[医药卫生—中药学]