希金斯炭疽菌自噬相关基因ChAtg3的功能研究  

Study on the functions of the autophagy related gene ChAtg3 in Colletotrichum higginsianum

作　　者：段灵涛 何九卿 王莉 陈炜伦 祝一鸣[1] 周而勋[1] DUAN Lingtao;HE Jiuqing;WANG Li;CHEN Weilun;ZHU Yiming;ZHOU Erxun(College of Plant Protection,South China Agricultural University/Guangdong Province Key Laboratory of Microbial Signals and Crop Disease Control,Guangzhou 510642,China)

机构地区：[1]华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室,广东广州510642

出　　处：《华南农业大学学报》2025年第2期175-185,共11页Journal of South China Agricultural University

基　　金：广东省自然科学基金(2021A1515011166);国家重点研发计划(2017YFD0200900)。

摘　　要：【目的】研究希金斯炭疽病菌Colletotrichum higginsianum中自噬相关基因ChAtg3的作用。【方法】对ChAtg3进行序列、系统发育和表达模式分析,并观察C.higginsianum侵染拟南芥Arabidopsis thaliana后ChAtg3的表达变化。通过根癌农杆菌介导的遗传转化技术,进行ChAtg3基因的敲除和回补。【结果】拟南芥接种C.higginsianum 8~40 h内ChAtg3基因表达量显著上升。敲除ChAtg3基因后的突变体(ΔChAtg3)自噬过程受抑制,其黑色素含量、产孢量、附着胞形成率和致病力均显著下降;但与野生型相比,菌丝生长和孢子萌发率并无显著变化。而通过基因回补,突变体CΔChAtg3在生物学表型和致病性上得以恢复。【结论】揭示了ChAtg3基因在希金斯炭疽病菌自噬过程、孢子产生和附着胞形成中的关键作用,是该病原菌致病力的关键调节因子。【Objective】To study the role of ChAtg3,an autophagy-related gene in Colletotrichum higginsianum.【Method】The sequence,phylogeny and expression pattern of ChAtg3 were analyzed,and the expression changes of ChAtg3 were observed in Arabidopsis thaliana after infection with C.higginsianum.An Agrobacterium tumefaciens-mediated genetic transformation system was employed to perform specific gene knockouts and their subsequent complementation.【Result】The expression changes of ChAtg3 were observed after C.higginsianum infected A.thaliana for 8 to 40 h.The mutantΔChAtg3,created through targeted ChAtg3 gene knockout,exhibited impaired autophagy,with markedly reduced melanin production,conidiation,appressorium formation,and pathogenic potential,although mycelial growth and conidial germination rate had no significant changes compared with wild type.Gene complementation in the CΔChAtg3 strain effectively reinstated its biological phenotype and pathogenicity.【Conclusion】The results reveal that ChAtg3 plays a key role in autophagy,conidiation,and appressorium formation of C.higginsianum,confirming it as a critical determinant of pathogenic capacity of C.higginsianum.

关 键 词：希金斯炭疽菌 自噬 ChAtg3基因 基因功能分析 致病力 

分 类 号：S432.44[农业科学—植物病理学]