猪肠病毒G型的分离鉴定及致病性研究  

Isolation and Characterization of Porcine Enterovirus Type G and Its Pathogenicity Study

作　　者：林艳 夏嘉鑫 周远成[2] 李敏 郑勤琴 陈莉群 岳丰雄 LIN Yan;XIA Jiaxin;ZHOU Yuancheng;L Min;ZHENG Qinqin;CHEN Liqun;YUE Fengxiong(Chengdu SG-Biotech Co.,Ltd..Chengdu 610100,China;Sichuan Provincial Key Laboratory of Livestock and Poutry Biological Products,Sichuan Animal Science Academy,Chengdu 610066,China;Chengdu Animal Disease Prevention and Control Center,Chengdu 610041,China)

机构地区：[1]成都史纪生物制药有限公司,四川成都610100 [2]四川省畜牧科学研究院动物营养健康研究所畜禽生物制品四川省重点实验室,四川成都610066 [3]成都市动物疫病预防控制中心,四川成都610041

出　　处：《中国猪业》2025年第1期63-72,共10页China Swine Industry

基　　金：四川省“十四五”川猪重大科技专项(2021ZDZX0010);四川省科技计划项目(2023YFN0021);“保育猪主要疫病防控关键技术创新及应用”(2024ZHCG0107)。

摘　　要：为分析临床腹泻发病猪场疫情发生的原因,在开展初生仔猪腹泻样品病毒分离试验中,获得了能够在Marc145细.胞上稳定增殖的病毒,并对该病毒进行宏基因组测序、核酸序列分析、培养特性研究、仔猪回归试验等研究。该病毒的分离株鉴定为猪肠病毒G1型(EV-G1),能在Marc145、ST、Vero等多种细胞上生长,无胰酶依赖性,对氯仿不敏感,适应细胞培养滴度均能达到107.0 TCIDg/mL以上。人工感染3~5日龄仔猪,可导致一过性腹泻,在感染仔猪的脑、心脏、空肠、回肠、盲肠、结肠中均可检测到该病毒。本研究从与猪轮状病毒混合感染的组织样品中分离到1株EV-G病毒,该病毒可导致新生仔猪腹泻,且病毒有良好的细胞适应性,旨在充分了解仔猪感染EV-G病毒的致病性,为EV-G免疫原性研究以及疫苗研发奠定基础。To analyze the causes of outbreaks in swine farms with clinical diarhea incidence.In conducting virus isolation experiments on diarhea samples from newborn piglets,viruses capable of stable proliferation on Marc 145 cells were obtained.The virus was also subjected to macro-genome sequencing.nucleic acid sequence analysis,culture characterization,and piglet regression testing.The isolate of this virus was identified as porcine enterovirus type G1(EV-G1).which could grow on a variety of cells including Marc145,ST,and Vero without trypsin dependence,was insensitive to chloroform,and was adapted to the cell culture titer could reach more than 1070 TCIDs/mL in all cases.Artificial infection of 3 to 5-day--old piglets could lead to transient diarhea,and the virus could be detected in the brain,heart,jejunum,ileum,cecum,and colon of infected piglets.In this study,one strain of EV-_G virus was isolated from tissue samples mixed with porcine rotavirus infection,which could cause diarrhea in newborn piglets,and the virus had good cellular adaptability,The aim was to fully understand the pathogenicity of EV-G virus infection in piglets and to lay the foundation for EV-G immunogenicity studies as well as vaccine development.

关 键 词：猪 仔猪 猪肠病毒G型 分离鉴定 生物学特性 人工感染模型 腹泻 

分 类 号：S828[农业科学—畜牧学] S858.28[农业科学—畜牧兽医]