Research Advances in the Construction and Application of Intestinal Organoids  

作　　者：Qingxue Meng Hongyang Yi Peng Wang Shan Liu Weiquan Liang Cuishan Chi Chenyu Mao Weizheng Liang Jun Xue Hongzhou Lu 

机构地区：[1]Technology Department,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China [2]Hebei Provincial Key Laboratory of Systems Biology and Gene Regulation,Central Laboratory,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China [3]National Clinical Research Centre for Infectious Diseases,The Third People’s Hospital of Shenzhen and The Second Affiliated Hospital of Southern University of Science and Technology,Shenzhen 518112,Guangdong,China [4]Hebei North University,Zhangjiakou 075000,Hebei,China [5]Bioimaging Core of Shenzhen Bay Laboratory,Shenzhen 518132,Guangdong,China [6]FAW Jiefang,Changchun 130011,Jilin,China [7]Huigu School of High-tech Industrial Development Zone,Changchun 130012,Jilin,China [8]University of Pennsylvania,PA 19104,United States [9]Hebei Provincial Key Laboratory of Systems Biology and Gene Regulation,Department of Surgery,The First Affiliated Hospital of Hebei North University,Zhangjiakou 075000,Hebei,China

出　　处：《Biomedical and Environmental Sciences》2025年第2期230-247,共18页生物医学与环境科学(英文版)

基　　金：funded by the National Natural Science Foundation of China(No.32300659);Shenzhen Science and Technology Innovation Commission Project(No.JCYJ20230807143302004);Zhangjiakou City Key R&D Plan Project(No.2421118D),Zhangjiakou City Key R&D Plan Project(No.2322088D);The Natural Science Project of Hebei North University(No.XJ2024034 and XJ2024035);Medical Science Research Subject Plan Project of Hebei Provincial Health Commission(No.20240782);Project of Administration of Traditional Chinese Medicine of Hebei Province(No.2025392);The 2025 Government-funded Training Project for Outstanding Clinical Medicine Talents(No.ZF2025264);Research Project of Medical Innovation for Chinese Youth(Project Leader:Jun Xue).g Project for Outstanding Clinical Medicine Talents(No.ZF2025264);Research Project of Medical Innovation for Chinese Youth(Project Leader:Jun Xue)。

摘　　要：The structure of intestinal tissue is complex.In vitro simulation of intestinal structure and function is important for studying intestinal development and diseases.Recently,organoids have been successfully constructed and they have come to play an important role in biomedical research.Organoids are miniaturized three-dimensional(3D)organs,derived from stem cells,which mimic the structure,cell types,and physiological functions of an organ,making them robust models for biomedical research.Intestinal organoids are 3D micro-organs derived from intestinal stem cells or pluripotent stem cells that can successfully simulate the complex structure and function of the intestine,thereby providing a valuable platform for intestinal development and disease research.In this article,we review the latest progress in the construction and application of intestinal organoids.

关 键 词：3D culture Colorectal cancer Disease model Intestinal organoids Organ transplantation Pluripotent stem cells Regenerative medicine Tumor organoids 

分 类 号：R318.1[医药卫生—生物医学工程]