丹参注射液对HBOC-CHP01复苏失血性休克大鼠肾脏保护作用的研究  

The renoprotective effect of Salvia miltiorrhiza injection on HBOC-CHP01 resuscitated haemorrhagic shock rats

作　　者：郝莎莎[1] 谢欣彤 李燊[1] 张鸿辉[1] 王红[1] 杨成民[1] 周文涛[1] 刘嘉馨[1] HAO Shasha;XIE Xintong;LI Shen;ZHANG Honghui;WANG Hong;YANG Chengmin;ZHOU Wentao;LIU Jiaxin(Institute of Blood Transfusion,Chinese Academy of Medical Sciences&Pecking Union Medical College,Chengdu 610052,China)

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052

出　　处：《中国输血杂志》2025年第2期172-180,共9页Chinese Journal of Blood Transfusion

基　　金：中国医学科学院医学与健康科技创新工程(2021-I2M-1-060);四川省中医药管理局面上项目(2023MS554)。

摘　　要：目的 探讨丹参注射液对HBOC-CHP01(聚合血红蛋白产品)复苏失血性休克大鼠肾脏的保护效果。方法 建立50%失血性休克大鼠模型,分成两组,丹参注射液(SMI)+HBOC-CHP01组和HBOC-CHP01组。SMI+HBOC-CHP01组大鼠在失血性休克后给予等失血量的HBOC-CHP01复苏,并给予8 mL/kg剂量的SMI,HBOC-CHP01组大鼠给予等失血量的HBOC-CHP01复苏,并给予8 mL/kg剂量的0.9%NaCl溶液。在大鼠放血前(baseline)、失血性休克(HS)、复苏后0点(RS 0 h)、复苏后1 h(RS 1 h)、复苏后24 h(RS 24 h),5个点取血,采用血气分析仪检测乳酸含量(Lac)、葡萄糖含量(Glu)、剩余碱(BEecf)、pH、碳酸氢根(HCO_(3)^(-))、高铁血红蛋白(MetHb);采用五分类检测白细胞(WBC)、血小板(PLT)、血红蛋白含量(Hb)、碳氧血红蛋白(COHb);采用生化仪检测肾脏相关指标血肌酐(SCr),尿酸(UA);在大鼠复苏24 h后,处死后取其肾脏组织,采用HE染色分析2组大鼠肾脏炎症情况,采用荧光染色法检测2组大鼠肾脏ROS水平。结果 在RS 0 h, SMI+HBOC-CHP01组大鼠的Beecf, Glu, Lac含量均显著低于HBOC-CHP01组大鼠,SMI+HBOC-CHP01组大鼠的pH水平显著高于HBOC-CHP01组大鼠,在RS 1 h, SMI+HBOC-CHP01组大鼠的Glu含量均显著低于HBOC-CHP01组大鼠;在RS 0 h, SMI+HBOC-CHP01组大鼠的WBC、 PLT和COHb含量均显著高于HBOC-CHP01组大鼠,在RS 1 h, SMI+HBOC-CHP01组大鼠的WBC含量显著高于HBOC-CHP01组大鼠;在RS 1 h, SMI+HBOC-CHP01组大鼠的UA含量显著低于HBOC-CHP01组大鼠,在RS 24 h, SMI+HBOC-CHP01组大鼠的SCr含量显著低于HBOC-CHP01组大鼠;在RS 24 h, SMI+HBOC-CHP01组大鼠的肾脏组织的炎症水平显著低于HBOC-CHP01组大鼠,SMI+HBOC-CHP01组大鼠的肾脏组织的ROS、MPO水平显著低于HBOC-CHP01组大鼠。结论 在HBOC-CHP01复苏重度失血性休克大鼠的过程中,结合使用丹参注射液,可以通过降低炎症反应和氧化应激缓解肾脏损伤。Objective To investigate the protective effect of Salvia miltiorrhiza injection(SMI)on the kidneys of HBOC-CHP01 resuscitated haemorrhagic shock rats.Methods A 50%haemorrhagic shock rat model was established,with 12 rats divided into two groups:SMI+HBOC-CHP01 group and HBOC-CHP01 group,with 6 rats in each group.The rats in the SMI+HBOC-CHP01 group were given an equal volume of HBOC-CHP01 for resuscitation after haemorrhagic shock,and an 8 mL/kg dose of SMI.Rats in the HBOC-CHP01 group were resuscitated by administering an equilibrium blood loss volume of HBOC-CHP01 and given an 8 mL/kg dose of 0.9%NaCl solution.Blood was taken from rats at five points:before bloodletting(baseline),during haemorrhagic shock(HS),immediately after resuscitation(RS0h),1 h after resuscitation(RS1h),and 24 h after resuscitation(RS24h).A blood gas analyser was used to detect the lactate level(Lac),glucose content(Glu),residual base(BEecf),pH,bicarbonate(HCO3-),high iron haemoglobin(MetHb).White blood cells(WBC),platelets(PLT),haemoglobin content(Hb),carboxyhaemoglobin(COHb)were detected using a quintuple classification.Blood creatinine(SCr),uric acid(UA),kidney-related indexes were detected using biochemistry instrument.Kidney tissues of the rats were taken after 24 h of resuscitation and after execution,and the inflammation of kidneys of the rats of the two groups was analyzed using HE staining.Fluorescence staining was used to detect the level of ROS in the kidneys of rats in both groups.Results At RS 0h,the Beecf,Glu and Lac levels of rats in the SMI+HBOC-CHP01 group were significantly lower than those of rats in the HBOC-CHP01 group,and the pH level of rats in the SMI+HBOC-CHP01 group was significantly higher than that of rats in the HBOC-CHP01 group,and the Glu levels of rats in the SMI+HBOC-CHP01 group were significantly lower than those of rats in the HBOC-CHP01 group at RS 1h.At RS 0h,the WBC,PLT and COHb contents of rats in the SMI+HBOC-CHP01 group were all significantly higher than those of rats in the HBOC-CHP01 group,and at

关 键 词：丹参注射液 HBOC-CHP01 复苏 失血性休克 肾脏保护 

分 类 号：R283[医药卫生—中药学] R541.64[医药卫生—中医学] R334.1R331.141