基于数字PCR方法的玉米内标准基因比较研究  

Comparison of Endogenous Genes in Maize Based on Digital PCR

作　　者：齐鑫 李欣然 郭亚宁 王丹 李凯 吴琼 李亮 QI Xin;LI Xinran;GUO Yaning;WANG Dan;LI Kai;WU Qiong;LI Liang(Institute of Quality Standard and Testing Technology for Agro-Products of Chinese Academy of Agricultural Sciences,Beijing 100081,China;College of Food Science and Bioengineering,Tianjin Agricultural University,Tianjin 300384,China;Beijing Genseed Technology Co.,Ltd.,Beijing 100194,China)

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081 [2]天津农学院食品科学与生物工程学院,天津300384 [3]北京创种科技有限公司,北京100194

出　　处：《生物技术进展》2025年第1期78-85,共8页Current Biotechnology

基　　金：农业生物育种国家科技重大专项(2022ZD04020)。

摘　　要：随着转基因玉米种类以及种植面积的不断增加,转基因玉米相关产品的定性和定量检测需求日益迫切。选择稳定遗传、特异性强的内标准基因对于转基因玉米鉴定结果的准确性和一致性具有重要意义。利用数字PCR技术对hmg、adh1-1、adh1-2、ivrI-1、ivrI-2、zSSIIb-1、zSSIIb-2、zein-1、zein-2在内的9种玉米内标准基因进行了筛选比较,通过退火温度的优化,找到各自最适的退火温度,并以转基因玉米MON810为研究对象,用上述内标准基因进行实际样品测定。结果表明,adh1-1和adh1-2可以用于实际样品的拷贝数检测。研究结果将为今后转基因玉米内标准基因的选择和相关定量检测提供参考,并对我国转基因作物的管理提供有力的技术支撑。With the increasing variety and planting area of transgenic maize,the demand for qualitative and quantitative detec⁃tion of transgenic maize-related products is increasingly urgent.The selection of stable genetic and specific endogenous genes is of great significance for the accuracy and consistency of transgenic maize identification results.In this study,digital PCR technol⁃ogy was used to screen and compare nine endogenous genes in maize,including hmg,adh1-1,adh1-2,ivrI-1,ivrI-2,zSSIIb-1,zSSIIb-2,zein-1 and zein-2.By optimizing the annealing temperature of the above nine kinds of endogenous genes,the optimal annealing temperature for each gene was found.Taking transgenic maize MON810 as the research object,the above endogenous genes were used to determine the actual samples.The results showed that adh1-1 and adh1-2 could be used for copy number de⁃tection of actual samples,which would provide reference for endogenous gene selection and related quantitative detection of trans⁃genic maize in the future,and provid a strong technical support for the management of transgenic crops in China.

关 键 词：转基因玉米 转基因检测 内标准基因 数字PCR 

分 类 号：Q812[生物学—生物工程] S513[农业科学—作物学]