新疆2种不同海拔绵羊EPAS1基因多态性与血液生理指标关联分析  

Association Analysis Between EPAS1 Gene Polymorphism and Blood Physiological Indicators in Two Different Altitudes of Sheep in Xinjiang

作　　者：董炅 王世锋 凯迪日耶·玉苏普 詹建立 阿布都热合曼·吐尔逊 DONG Jiong;WANG Shifeng;YUSUPU·Kaidiriye;ZHAN Jianli;TUERXUN·Abudureheman(Key Laboratory of Biological Resources and Ecology of Pamirs Plateau Xinjiang Uygur Autonomous Region,the College of Life and Geographic Sciences,Kashi University,Xinjiang Kashi 844000,China)

机构地区：[1]喀什大学生命与地理科学学院,新疆帕米尔高原生物资源与生态重点实验室,新疆喀什844000

出　　处：《生物技术进展》2025年第1期119-126,共8页Current Biotechnology

基　　金：新疆高校科研计划项目(XJEDU2021SI024)。

摘　　要：为探究不同海拔新疆地方绵羊EPAS1基因多态性与血红蛋白指标及低氧适应的关联,对79只塔什库尔干羊(海拔4200 m)和74只多浪羊(海拔1200 m)EPAS1基因的第9、第16外显子进行PCR扩增并测序。共检测到7个SNP位点,随后计算基因频率和基因型频率,并分析了血红蛋白指标的差异。结果表明,塔什库尔干羊的血红蛋白含量极显著高于多浪羊,这表明塔什库尔干羊通过增加血红蛋白含量来适应高海拔引起的低氧环境。同时,EPAS1基因的第9、第16外显子相对保守,氨基酸水平的变异远低于核苷酸水平。此外,多浪羊的遗传信息更为多样,显示出海拔与多态信息含量呈负相关趋势,为进一步理解塔什库尔干羊适应高海拔低氧环境的分子基础和血液生理指标变化提供了参考。To explore the relationship between EPAS1 gene polymorphisms and hemoglobin indices in hypoxia adaptation among local Ovis aries populations in Xinjiang at varying altitudes,exons 9 and 16 of the EPAS1 gene were PCR-amplified and se⁃quenced in 79 Tashkurgan sheep(4200 m altitude)and 74 Duolang sheep(1200 m altitude).7 SNP loci were identified,gene frequencies and differences in hemoglobin indices were analyzed.The results revealed that the hemoglobin content in Tashkurgan sheep was significantly higher than in Duolang sheep,suggesting that Tashkurgan sheep adapt to high-altitude hypoxia by in⁃creasing hemoglobin levels.Additionally,exons 9 and 16 of the EPAS1 gene were relatively conserved,with amino acid-level variation being significantly lower than nucleotide-level variation.Duolang sheep exhibited greater genetic diversity at the same polymorphic loci,indicating a negative correlation between altitude and polymorphic information content.These findings provide insights into the molecular mechanisms underlying Tashkurgan sheep′s adaptation to high-altitude hypoxia and changes in blood physiological indices.

关 键 词：EPAS1基因 低氧适应 多态性 血红蛋白 

分 类 号：Q78[生物学—分子生物学] S826[农业科学—畜牧学]