硫酸阿托品对光学离焦性近视豚鼠近视控制及视网膜内血管活性肠肽、多巴胺水平的影响  

Effects of atropine sulfate on myopia control and the levels of vasoactive intestinal peptide and dopamine in the retinae of guinea pigs with lens-induced myopia

作　　者：符俊达 朱海萍[1] 张涵瑜 杨婉晨 孙轶军[1] FU Junda;ZHU Haiping;ZHANG Hanyu;YANG Wanchen;SUN Yijun(Ophthalmology Department,Qinhuangdao First Hospital,Qinhuangdao 066000,Hebei,China)

机构地区：[1]秦皇岛市第一医院眼科,河北秦皇岛066000

出　　处：《中南医学科学杂志》2025年第1期28-31,73,共5页Medical Science Journal of Central South China

基　　金：秦皇岛市科学技术研究与发展计划项目(202301A190)。

摘　　要：目的观察0.01%硫酸阿托品对光学离焦性近视(LIM)豚鼠近视控制及视网膜内血管活性肠肽(VIP)、多巴胺(DA)水平的影响。方法选取豚鼠30只,随机均分为阿托品1组(每日滴用硫酸阿托品1次)、阿托品2组(每日滴用硫酸阿托品2次)、对照组(每日滴用0.3%玻璃酸钠2次)。比较各组干预前后屈光度、眼轴长度、巩膜厚度变化,并比较各组干预后视网膜的结构、细胞凋亡数量、VIP、DA水平。结果与干预前比较,各组干预后屈光度、眼轴长度均增大,巩膜厚度均变薄;上述趋势阿托品1组、阿托品2组小于对照组(P<0.05),而阿托品1组与阿托品2组比较差异无显著性(P>0.05)。硫酸阿托品干预后,未造成LIM豚鼠视网膜病理变化加重;阿托品1组、阿托品2组视网膜细胞凋亡数量、视网膜VIP阳性细胞数低于对照组,DA含量高于对照组(P<0.05),阿托品2组与阿托品1组比较差异无显著性(P>0.05)。结论0.01%硫酸阿托品滴眼液不同用药频次均能下调LIM豚鼠视网膜内VIP,上调DA表达,减缓眼轴增长速度,抑制近视进展速度,两种用药方式效果相当。Aim To observe the effects of 0.01%atropine sulfate on myopia control and the levels of vasoactive intestinal peptide(VIP)and dopamine(DA)in the retinae of guinea pigs with lens-induced myopia(LIM).Methods Thirty guinea pigs were selected.They were randomly and equally divided into atropine group 1(administrated with atropine sulfate once a day),atropine group 2(administrated with atropine sulfate twice a day),and control group(administrated with 0.3%sodium hyaluronate twice a day).The three groups were compared in terms of changes in diopter,axial length and scleral thickness before and after intervention,as well as retinal structure,cell apoptosis rate,and the levels of VIP and DA after intervention.Results Compared with the levels before intervention,after intervention,the refractive error and axial length of the eyes increased in all groups,and the thickness of the sclera decreased;the above data showed a trend that atropine group 1 and atropine group 2 were smaller than the control group(P<0.05),but there was no significant difference between group 1 and group 2 of atropine(P>0.05).After intervention with atropine sulfate,there was no aggravation of retinal pathological changes in LIM guinea pigs;the number of apoptotic retinal cells and VIP positive retinal cells in atropine group 1 and atropine group 2 were lower than those in the control group,and the DA content was higher than that in the control group(P<0.05).But there was no significant difference between atropine group 2 and atropine group 1(P>0.05).Conclusion Administration with 0.01%atropine sulfate eye drops at different frequencies all can downregulate VIP expression and upregulate DA expression in the retinae of guinea pigs with LIM.In addition,they can inhibit the increase of ocular axis and the progression of myopia,with comparable effects.

关 键 词：硫酸阿托品 光学离焦性近视 近视控制 血管活性肠肽 多巴胺 

分 类 号：R988.1[医药卫生—药品]