MALDI-TOF MS在佩戴过的口罩细菌污染情况研究中的应用  

Application of MALDI-TOF MS in the study of bacterial contamination of worn masks

作　　者：丁国涛 杨国兴[1] 赵艳 方彦飞[1] 高腾 李伟昊[1] DING Guotao;YANG Guoxing;ZHAO Yan;FANG Yanfei;GAO Teng;LI Weihao(Department of Physical and Chemical and Microbiology Clinical Laboratory,Handan Municipal Center for Disease Control and Prevention,Handan,Hebei 056000,China)

机构地区：[1]邯郸市疾病预防控制中心理化微生物检验科,河北邯郸056000

出　　处：《医学动物防制》2025年第1期35-39,共5页Journal of Medical Pest Control

基　　金：河北省医学科学研究课题计划(20220512)。

摘　　要：目的应用基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption ionization time-of-flight mass spectrometry,MALDI-TOF MS)分析不同佩戴时长口罩的细菌污染情况。为相关部门提出合理的口罩佩戴时长提供依据。方法于2022年7月招募志愿者在日常工作环境中佩戴口罩,佩戴时长为0.5h、1.0h、2.0h、4.0h、5.0h;使用倾注平板法测定不同佩戴时长口罩内外表面的细菌总数;使用MALDI-TOF MS方法鉴定并分析口罩内外表面细菌丰度。统计学分析采用校正的F检验方法。结果佩戴5.0h口罩内表面与外表面的细菌总数差异有统计学意义[平均值差值(mean deviation,MD)=380.9,P<0.001];佩戴0.5h、1.0h、2.0h和4.0h口罩内表面与外表面的细菌总数差异无统计学意义(MD=9.2,P=0.704;MD=9.3,P=0.701;MD=40.2,P=0.099;MD=19.0,P=0.433)。整个研究共分离320个单菌落。佩戴4.0h的口罩内外表面同时检出的菌属数为2,穿透率最高为33.3%。佩戴5.0h的口罩内外表面同时检出的菌属数为5,穿透率为25.0%~100.0%。结论随佩戴时间延长,口罩内外表面细菌总数增加。同一佩戴时长,口罩内表面的细菌数量高于口罩外表面;同一佩戴时长,口罩内表面的细菌种类低于口罩外表面。建议佩戴时长超过4.0h更换口罩。Objective To analyze the bacterial contamination of masks with different wearing times by the matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS),and to provide a basis for the relevant departments to propose a reasonable wearing time of masks.Methods In July 2022,volunteers were recruited to wear masks in their daily work environments for wearing times of 0.5h,1.0h,2.0h,4.0h and 5.0h,respectively.The total bacterial count on the inner and outer surfaces of the masks at different wearing times was determined by the pour plate method;the bacterial abundance on the inner and outer surfaces of the masks was identified and analyzed by the MALDI-TOF MS method.Statistical analyses were performed using the adjusted F-test method.Results There was a statistically significant difference in the total bacterial counts between the inner surface and the outer surface of the mask worn for 5.0h[mean deviation(MD)=380.9,P<0.001].There was no statistically significant difference in the total bacterial counts between the inner surface and the outer surface of the masks worn for 0.5h,1.0h,2.0h and 4.0h(MD=9.2,P=0.704;MD=9.3,P=0.701;MD=40.2,P=0.099;MD=19.0,P=0.433).A total of 320 single colonies were isolated throughout the study.The number of bacterial genera detected simultaneously on the inner and outer surfaces of masks worn for 4.0h was 2,with a top penetration rate of 33.3%.The number of bacterial genera detected simultaneously on the inner and outer surfaces of masks worn for 5.0h was 5,with the penetration rate of 25.0%to 100.0%.Conclusion The total bacterial count on both the inner and outer surfaces of the masks increases with longer wearing times.The number of bacteria on the inner surface of the mask is higher than that on the outer surface of the mask for the same wearing time,and the bacterial species on the inner surface of the mask is lower than that on the outer surface.It is recommended to replace the mask when wearing it for more than 4.0h.

关 键 词：基质辅助激光解析电离飞行时间质谱 佩戴时长 细菌污染 细菌丰度 细菌穿透率 

分 类 号：E446.5[军事—军事理论]