白术内酯I减轻急性心肌梗死大鼠炎性反应的影响  

Atractylenolide I Attenuates Inflammatory Response in Rats with Acute Myocardial Infarction

作　　者：曾玉英[1] 姜花[1] 唐万云 无 ZENG Yuying;JIANG Hua;TANG Wanyun(Qinghai Red Cross Hospital,Qinghai Xining 810099,China)

机构地区：[1]青海红十字医院,青海西宁810099 [2]青海省中医院肺病科,青海西宁810000

出　　处：《河北医学》2025年第2期245-251,共7页Hebei Medicine

基　　金：青海省卫生健康委科研项目,(编号:2023-wjzd-12)。

摘　　要：目的:探讨白术内酯I(ATR-I)对急性心肌梗死(AMI)大鼠炎性反应的影响。方法:将大鼠分为假手术组、AMI组、ATR-Ⅰ-L剂量组、ATR-Ⅰ-M剂量组、ATR-Ⅰ-H剂量组和ATR-Ⅰ-H+普乐沙福(AMD3100)干预模型组,每组12只。每天给药1次,持续7d。超声影像系统测量大鼠左室短轴缩短率(FS)和射血分数(LVEF);TTC染色检测大鼠心肌梗死面积;HE染色检测大鼠心肌组织病理学;TUNEL染色检测大鼠心肌细胞凋亡;ELISA试剂盒检测大鼠心肌组织中乳酸脱氢酶(LDH)活性、肌红蛋白(MB)、白细胞介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)水平;RT-qPCR检测大鼠组织中SDF-1和CXCR4 mRNA的相对表达量;Western blot测定心肌组织中SDF-1和CXCR4蛋白表达。结果:与假手术组相比,AMI组大鼠LVSF和FS、心肌组织中SDF-1、CXCR4 mRNA及其蛋白表达下降(P<0.05),心肌梗死面积、心肌细胞凋亡率和心肌组织中LDH活性、MB、IL-1β和TNF-α水平上升(P<0.05),心肌细胞损伤严重伴有炎性发生;与AMI组相比,ATR-Ⅰ-L组、ATR-Ⅰ-M组和ATR-Ⅰ-H组大鼠LVSF和FS、心肌组织中SDF-1、CXCR4 mRNA及其蛋白表达升高(P<0.05),心肌梗死面积、心肌细胞凋亡率和心肌组织中LDH活性、MB、IL-1β和TNF-α水平降低(P<0.05),心肌细胞损伤有所改善;与ATR-Ⅰ-H组相比,ATR-Ⅰ-H+AMD3100组大鼠LVSF和FS、心肌组织中SDF-1、CXCR4 mRNA及其蛋白表达降低(P<0.05),心肌梗死面积、心肌细胞凋亡率和心肌组织中LDH活性、MB、IL-1β和TNF-α水平上升(P<0.05),心肌细胞损伤加剧。结论:ATR-I减轻AMI大鼠炎性反应。Objective:To investigate the effect of atractylenolide I(ATR-I)on inflammatory response in acute myocardial infarction(AMI)rats.Methods:The rats were divided into a sham operation group,an AMI group,an ATR-Ⅰ-L dose group,an ATR-Ⅰ-M dose group,an ATR-Ⅰ-H dose group,and an ATR-Ⅰ-H+plerixafor(AMD3100)model group,with 12 rats in each group,once a day for 7 days.The ultrasound imaging system was applied to measure fraction shorting(FS)and left ventricular ejection fraction(LVEF)in rats.The myocardial infarction area,myocardial histopathology,and cardiomyocyte apoptosis in rats were detected by 2,3,5-triphenyl tetrazolium chloride(TTC)staining,hematoxylin-eosin(HE)staining,and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)staining,respectively.The changes in lactate dehydrogenase(LDH),myoglobin(MB),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)in myocardial tissue were calculated by enzyme-linked immunosorbent assay(ELISA).Reverse transcription-quantitative polymerase chain reaction(RT-qPCR)and Western blot were employed to determine the expression levels of stromal cell-derived factor 1(SDF-1)and C-X-C chemokine receptor type 4(CXCR4),and the proteins expression of SDF-1 and CXCR4 mRNA in rat tissues.Results:Compared with the sham surgery group,the levels of LVSF and FS,and the expression of SDF-1,CXCR4 mRNA and their protein expression in myocardial tissue of AMI group decreased significantly(P<0.05).The myocardial infarction area,apoptosis rate,and levels of LDH activities,MB,IL-1β,and TNF-α in myocardial tissue increased significantly(P<0.05),with severe myocardial cell damage and inflammation.Compared with the AMI group,the levels of LVSF and FS,and the expression of SDF-1,CXCR4 mRNA and their protein expression in myocardial tissue of ATR-I-L group,ATR-I-M group,and ATR-I-H group increased significantly(P<0.05).The myocardial infarction area,apoptosis rate,and levels of LDH activities,MB,IL-1β,and TNF-α in myocardial tissue decreased significantly(P<0.05),th

关 键 词：白术内酯I 基质细胞衍生因子-1 CXC趋化因子受体4 急性心肌梗死 炎性反应 

分 类 号：R285.5[医药卫生—中药学]