Antidiabetic and antioxidant properties of Fagara zanthoxyloides root-bark ethyl acetate fraction:in-vitro enzyme inhibition and GC-MS analysis  

作　　者：Christian Amah Parker Joshua Ikechukwu Okoro Favour Iyidiegwu Chibuike Brendan Linus Nsude Ugochukwu Nweke 

机构地区：[1]Department of Biochemistry,Faculty of Natural and Applied Sciences,State University of Medical and Applied Sciences,Nsukka 412111,Nigeria [2]Department of Biochemistry,Faculty of Biological Sciences,University of Nigeria,Nsukka 410001,Nigeria [3]Department of Medical Biochemistry,College of Health Sciences,David Umahi Federal University of Health Sciences,Uburue,Uburu 491105,Nigeria [4]Department of Chemical Sciences,Faculty of Natural and Applied Sciences,Spiritan University Nneochi,Nneato Road,Leru 441111,Nigeria [5]Department of Genetics and Biotechnology,Faculty of Natural and Applied Sciences,State University of Medical and Applied Sciences Igbo-Eno,Nsukka 412111,Nigeria [6]Department of Pharmacognosy and Natural Medicine,University of Uyo,Uyo 520003,Nigeria

出　　处：《Life Research》2025年第1期32-43,共12页TMR生命研究

摘　　要：The application of therapeutic herbs in treatment of common infections and diseases has gained popularity in developing nations due to its cost-effectiveness and purported lower side effects compared to synthetic drugs.Fagara zanthoxyloides Lam also known as Zanthoxylum zanthoxyloides,is an indigenous plant used widely as chewing stick for tooth cleaning in West Africa.The root-bark macerations,decoctions or infusions are the most valuable organs widely taken to treat malaria and diabetes by local populations,traditional healers and local pharmaceutical firms.Searching alternative,more efficient,safer,and less expensive anti-diabetic natural products from plants has received great attention,hence this research delved into the inhibitory effects of the ethyl acetate fraction of F.zanthoxyloides root-bark(EAFFZRB)on in-vitroα-amylase andα-glucosidase enzymes.Standard biochemical methods were used to extract and assess the in-vitro antioxidant such as 2,2-diphenyl-1-picrylhydrazl(DPPH),total antioxidant capacity(TAC)and Ferric Reducing Antioxidant Power(FRAP).The sample was combined at different concentrations(10,20,40,80,160,320,and 640μl/dl).Theα-amylase,andα-glucosidase activities of EAFFZRB sample was combined at different concentrations(0.2,0.4,0.6,0.8 and 1.0 mg/mL).The phyto-constituents in EAFFZRB were identified using Gas Chromatography-Mass Spectrometry(GC-MS)technique.The plant fraction showed a significant(P<0.05)increase in the inhibition ofα-amylase IC50=0.726 andα-glucosidase IC50=0.877 at varying concentrations,comparable to the reference drug Acarbose IC50=0.728 and IC50=0.852 respectively.EAFFZRB demonstrated significant(P<0.05)inhibition at different concentrations on DPPH(66.55±1.57),TAC(0.47±0.17)and FRAP(3.64±0.14),similar to the effect of the standard ascorbic acid.The GC-MS analysis of EAFFZRB revealed a total of 12 volatile organic compounds such as;Phenol-3-methoxy-2,5,6-trimethyl,2-Hydroxy-5-nitrobenzylbromide,2,6-Dimethyl-4-(3-nitrophenyl)pyridine,1H-Benz[e]indene,2-methyl and o

关 键 词：Fagara zanthoxyloides α-amylase andα-glucosidase ANTI-OXIDANT GC-MS 

分 类 号：R28[医药卫生—中药学]