目标温度管理对热射病大鼠肺细胞焦亡的影响  

ffect of target temperature management on pyroptosis of lung cells in heat stroke rats

作　　者：吴长东[1] 王彬 唐佳 热夏提江·库热西 穆叶赛·尼加提[1] WU Changdong;WANG Bin;TANG Jia;Rexiatijiang·Kurexi;Muyesai·Nijiati(First Aid Centre,Xinjiang Uygur Autonomous Region People’s Hospital,Urumqi,Xinjiang,830000,China;Graduate School,Xinjiang Medical University,Urumqi,Xinjiang,830001,China)

机构地区：[1]新疆维吾尔自治区人民医院急救中心,乌鲁木齐830000 [2]新疆医科大学研究生院,乌鲁木齐830001

出　　处：《重庆医学》2025年第2期313-318,共6页Chongqing Medical Journal

基　　金：新疆维吾尔自治区自然科学基金项目(2020D01C106)。

摘　　要：目的 通过不同目标温度降温干预经典热射病(CHS)大鼠,比较肺细胞焦亡指标的变化,探讨经典热射病适宜的目标温度选择。方法 将雄性SD大鼠40只按随机数字表法分为空白对照组(n=10)和经典热射病组(CHS组,n=30),CHS组按照大鼠初始核心温度(Tic)±1℃,分为CHS0组(Tic),CHS1组(Tic-1℃)和CHS2组(Tic+1℃)。将CHS组大鼠麻醉后俯卧置于热环境模拟舱,当核心体温达到42.0℃建立热打击大鼠模型。建模成功后立即使用室温水浴降温,降至该组设定的目标温度后,使用降温毯维持3 h。空白对照组始终置于室温环境。实验结束后收集大鼠股动脉血标本,通过酶联免疫吸附试验(ELISA)检测大鼠血清白细胞介素(IL)-1β、IL-18水平;取大鼠肺标本,制备组织匀浆,采用ELISA检测肺组织IL-1β、IL-18水平及采用Western blot法检测Gasdermin D(GSDMD)、半胱氨酸天冬氨酸蛋白酶-1前体(pro-caspase-1)蛋白表达水平,按照公式或标准曲线计算标本中酶的活性。结果 CHS0组大鼠肺组织及血清IL-1β、IL-18水平,CHS1组血清IL-18水平高于空白对照组(P<0.05)。CHS1组肺组织IL-1β水平,CHS2组IL-1β、IL-18水平,CHS1、CHS2组血清IL-18水平低于CHS0组(P<0.05),CHS1与CHS2组间比较差异无统计学意义(P>0.05)。CHS0、CHS2组GSDMD、pro-caspase-1水平,CHS1组GSDMD水平高于空白对照组(P<0.05)。CHS1、CHS2组GSDMD、pro-caspase-1水平低于CHS0组,CHS1组pro-caspase-1水平低于CHS2组(P<0.05)。结论 热打击大鼠肺细胞焦亡效应分子及蛋白表达水平增高,降温干预可减轻其肺组织细胞焦亡。设定目标温度为高于或低于初始核心温度一定范围的降温干预,可能对热打击大鼠肺组织具有更好的保护作用。Objective To compare the changes of lung cell pyroptosis indexes in heat stress rats through different target temperature cooling intervention on classical heat stroke(CHS)rats,and to explore the choice of suitable target temperature of CHS.Methods A total of 40 male SD rats were divided into the blank control group(n=10)and classical heat stroke(CHS,n=30)group according to the random number table method.The CHS group was divided into the CHS0 group(Tic),CHS1 group(Tic-1℃)and CHS2 group(Tic+1℃)according to the initial core temperature(Tic)±1℃.After anesthesia,the rats in CHS group were placed by the prone position in the thermal environment simulation chamber,and the heat shock rat model was established when the core body temperature reached 42℃.Immediately after successful modeling,the room temperature water bath was used to cool down to the setting target temperature of the group,and then the cooling blanket was used to maintain the body temperature for 3 h.The blank control group was always kept at room temperature.At the end of the experiment,the blood samples of femoral artery of rats were collected,and the levels of serum interleukin-1β(IL-1β)and interleukin-18(IL-18)were detected by ELISA.The lung samples of rats were taken to prepare the tissue homogenate.Then the concentrations of IL-1βand IL-18 in the lung tissue were detected by ELISA and Western blot method was used to detect GSDMD and cysteine aspartate protease-1 precursor(pro-caspase-1)protein expression levels in rat lung tissue,and the enzyme activity in the sample was calculated according to the formula or standard curve.Results The levels of IL-1βand IL-18 in lung tissues and serum in the CHS0 group and serum IL-18 level in the CHS1 group were higher than those in the blank control group(P<0.05).The lung tissue IL-1βlevel in the CHS1 group,the IL-1βand IL-18 levels in the CHS2 group,and the IL-18 level in the serum CHS1 and CHS2 groups were lower than those in the CHS0 group.There was no statistically significant difference betwe

关 键 词：热射病 目标温度管理 细胞焦亡 GSDMD 半胱氨酸天冬氨酸蛋白酶-1前体 

分 类 号：R818[医药卫生—放射医学]