灵芝醇提物对APP/PS1小鼠肠道菌群组成及多样性的影响  

Effects of Ganoderma lucidum alcohol extract on composition and diversity of intestinal flora in APP/PS1 mice

作　　者：傅伟淇 邹师琪 谭锻玲 杨欣[1,2,3] FU Wei-Qi;ZOU Shi-Qi;TAN Duan-Ling(The Fifth Clinical College of Guangzhou Medical University,Guangzhou 510070,Guangdong,China)

机构地区：[1]广州医科大学第五临床学院,广东广州510070 [2]广州医科大学附属第五医院 [3]广州医科大学药学院

出　　处：《吉林医学》2025年第3期513-517,共5页Jilin Medical Journal

基　　金：广州市卫生健康委员会中医药与中西医结合项目[项目编号:20212A011027];2023年度高水平大学“一流本科专业建设经费”资助项目[项目编号:2022JXA008];广州市科技局市校(院)企联合资助项目[项目编号:2024A03J0072]。

摘　　要：目的:采用高通量测序研究灵芝醇提物对APP/PS1小鼠肠道菌群多样性及组成的影响。方法:APP/PS1小鼠随机分为模型组、灵芝组,每组10只,C57BL/6J小鼠为对照组(n=10),模型组和对照组用灵芝组等体积蒸馏水处理持续6个月,行为学评价后采集各组小鼠新鲜粪便样本,进行微生物群落多样性测序分析,比较各组小鼠肠道菌群组成和结构。结果:灵芝组与模型组小鼠肠道菌群组成存在显著差异。与模型组比较,灵芝组Akkermansia,Enterobacter,Coprobacillus、Clostridium丰度显著增高,差异有统计学意义(P<0.05),Adlercreutzia,Alistipes,Anaerotruncus,Bacillus丰度显著降低,差异有统计学意义(P<0.05)。结论:灵芝醇提物可增加APP/PS1小鼠肠道Akkermansia、Enterobacter、Coprobacillus、Clostridium等有益菌群的丰度,降低肠道Adlercreutzia、Alistipes、Anaerotruncus、Bacillus等有害菌群丰度,影响小鼠肠道菌群组成与比例。Objective To study the effects of Ganoderma lucidum alcohol extract on intestinal flora diversity and composition in APP/PS1 mice using high-throughput sequencing.Methods APP/PS1 mice were randomly divided into model group,Ganoderma lucidum group,and control group(consisting of C57BL/6J mice).Each group contained 10 animals.The model group and control group were administered equal volumes of distilled water,while the Ganoderma lucidum group received the extract.After 6 months of treatment,behavioral evaluations were conducted.Fresh fecal samples were collected from mice in each group,and microbial community diversity sequencing and interactive analysis were performed.The results were analyzed using bioinformatics software to compare the composition and structure of intestinal flora among the groups.Results Significant differences in intestinal flora were observed between the Ganoderma lucidum group and the model group.Compared with the model group,the abundances of Akkermansia,Enterobacter,Coprobacillus,and Clostridium increased significantly(P<0.05),while those of Adlercreutzia,Alistipes,Anaerotruncus,and Bacillus decreased significantly(P<0.05)in the Ganoderma lucidum group.Conclusions The Ganoderma lucidum alcohol extract could increase the abundance of certain beneficial bacteria and decrease the abundance of certain harmful bacteria in the intestinal flora of mice,thereby affecting the composition and proportion of the intestinal flora.

关 键 词：灵芝醇提物 肠道菌群 高通量测序 

分 类 号：R285[医药卫生—中药学]