3.0 T磁共振成像评估大鼠自身免疫性前列腺炎的实验性研究  

An experimental study of 3.0 T magnetic resonance imaging to evaluate autoimmune prostatitis in rats

作　　者：陈子琦 李宜泽 单春辉 俞秀巧 张璐瑶 王振显[1] 陈英敏[3] CHEN Ziqi;LI Yize;SHAN Chunhui;YU Xiuqiao;ZHANG Luyao;WANG Zhenxian;CHEN Yingmin(Department of Urology,Hebei General Hospital,Shijiazhuang 050051,China;Graduate School of Hebei Medical University,Shijiazhuang 050017,China;Department of Medical Imaging,Hebei General Hospital,Shijiazhuang 050051,China;Department of Ultrasound Medicine,Hebei General Hospital,Shijiazhuang 050051,China)

机构地区：[1]河北省人民医院泌尿外科,石家庄050051 [2]河北医科大学研究生学院,石家庄050017 [3]河北省人民医院医学影像科,石家庄050051 [4]河北省人民医院超声医学科,石家庄050051

出　　处：《磁共振成像》2025年第2期100-106,共7页Chinese Journal of Magnetic Resonance Imaging

基　　金：2020年度河北省政府资助临床医学优秀人才培养项目(编号:冀卫办科教〔2021〕1号)。

摘　　要：目的探讨3.0 T MRI在评估大鼠实验性自身免疫性前列腺炎(experimental autoimmune prostatitis,EAP)严重程度及补锌治疗效果中的应用价值。材料与方法本研究为前瞻性随机对照实验,以传统的自身免疫性前列腺炎大鼠的建模方式为基础构建5组大鼠模型,分别为对照(normal control,NC)组(6只)、模型组A(EAP-A组)(6只)、治疗组A(EAPZ-A组)(6只)、模型组B(EAP-B组)(6只)以及治疗组B(EAPZ-B组)(6只)。其中,EAP-A组与EAPZ-A组大鼠采用15 mg/mL异体前列腺抗原混合物皮内注射进行造模,EAP-B组与EAPZ-B组大鼠采用30 mg/mL异体前列腺抗原混合物皮内注射进行造模,NC组大鼠则采用等量的生理盐水皮内注射进行造模。在模型构建前(12周龄)、模型构建后(18周龄)及治疗组补锌结束后(22周龄)对各组大鼠行MRI扫描,包括轴位T1WI、T2WI、弥散加权成像(diffusion weighted imaging,DWI)和矢状位质子密度加权成像(proton density weighted imaging,PDWI);在22周龄磁共振扫描结束后取材,行苏木精-伊红(hematoxylin-eosin,HE)病理染色进行观察。测量各组大鼠前列腺在不同周龄的标准化T2WI信号强度及平均扩散系数(average diffusion coefficient,ADC)值,并行组间及组内两两比较。其中,各组大鼠前列腺在不同周龄的标准化T2WI信号强度的组间比较采用单因素方差分析,当P<0.05时进一步开展最小显著差异(least significant difference,LSD)多重比较;各组大鼠前列腺在不同周龄的标准化T2WI信号强度的组内比较采用配对样本t检验。各组大鼠前列腺在不同周龄的ADC值的组间比较采用单因素方差分析;各组大鼠前列腺在不同周龄的ADC值的组内比较采用配对样本t检验。结果12周龄时,各组之间的标准化T2WI信号强度差异均无统计学意义(P=0.918)。18周龄建模成功后,除NC组的标准化T2WI信号强度未下降外,其余四组均不同程度降低,且EAP-A组/EAPZ-A组的标准化T2WI信号强度均高于EAPObjective:To explore the application value of 3.0 T MRI in evaluating the severity of experimental autoimmune prostatitis(EAP)and the effect of zinc supplementation in rats.Materials and Methods:This study was a prospective randomized controlled experiment,and five groups of rat models were constructed based on the traditional modeling method of autoimmune prostatitis rats,which were normal control(NC)group(6 rats),model group A(EAP-A group)(6 rats),treatment group A(EAPZ-A group)(6 rats),model group B(EAP-B group)(6 rats),and treatment group B(EAPZ-B group)(6 rats).Among them,the rats in the EAP-A group and the EAPZ-A group were modeled by intradermal injection of 15 mg/mL allogeneic prostate antigen mixture,the rats in the EAP-B group and the EAPZ-B group were modeled by intradermal injection of 30 mg/mL allogeneic prostate antigen mixture,and the rats in the NC group were modeled by intradermal injection of the same amount of normal saline.MRI scans were performed before the model was constructed(12 weeks old),after the model was constructed(18 weeks old)and after the treatment group was supplemented with zinc(22 weeks old),including axial T1WI,T2WI,diffusion weighted image(DWI)and sagittal proton density-weighted imaging(PDWI).At the end of the 22-week-old scan,the samples were collected and observed by pathological staining of hematoxylin-eosin(HE).The standardized T2WI signal intensity and average diffusion coefficient(ADC)values of the prostate gland at different weeks in each group were measured,and the prostate was compared between and within groups.Among them,one-way ANOVA was used to compare the standardized T2WI signal intensity of the prostate gland at different weeks in each group,and the least significant difference(LSD)was further compared when P<0.05.The paired t-test was used for intra-group comparison of standardized T2WI signal intensity of rat prostate at different weeks of age in each group.One-way ANOVA was used to compare the ADC values of the prostate gland at different weeks in each grou

关 键 词：实验性自身免疫性前列腺炎 Wistar大鼠模型 磁共振成像 锌 疗效评价 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]