基于超小超顺磁性纳米氧化铁增强MRI评价1型糖尿病兔骨髓H型血管  

Evaluation of type-H vessels in bone marrow of type 1 diabetic rabbits based on ultra-small superparamagnetic nano-iron oxide enhanced MRI

作　　者：雷浩然 王克军[1] 李亮[1] 刘昌盛[1] 查云飞[1] LEI Haoran;WANG Kejun;LI Liang;LIU Changsheng;ZHA Yunfei(Department of Radiology,Renmin Hospital of Wuhan University,Wuhan 430060,China)

机构地区：[1]武汉大学人民医院放射科,武汉430060

出　　处：《磁共振成像》2025年第2期107-113,共7页Chinese Journal of Magnetic Resonance Imaging

基　　金：国家自然科学基金项目(编号:82171895,81871332)。

摘　　要：目的探索基于超小超顺磁性纳米氧化铁(ultra-small superparamagnetic nano-iron oxide,USPIO)增强MRI联合离体显微CT(micro-computed tomography,Micro-CT)微血管成像定量可视化靶向评价1型糖尿病(type 1 diabetes mellitus,T1DM)兔骨髓H型血管结构的可行性。材料与方法本研究于2023年9月至2024年4月开展,从40只2~3月龄日本大耳白兔中随机选取20只兔耳缘静脉注入四氧嘧啶构建T1DM兔,其余兔注射相同剂量生理盐水作为对照组,于造模成功4个月后分别取T1DM组和对照组兔的胫骨干骺端和骨干部位样本进行骨髓H型血管内皮细胞分选与鉴定、H型血管免疫荧光检测、骨髓血管内皮细胞体外与体内USPIO标记与MRI以及离体骨髓Micro-CT微血管成像,并分别测量H型血管内皮细胞占比、平均荧光强度、MRI的T2值及血管体积分数(vessel volume/tissue volume,VV/TV)和血管数量(vessel number,VN)等定量参数结果。结果T1DM组兔相较于对照组兔,骨髓H型血管内皮细胞占比、平均荧光强度、VV/TV、VN都存在明显降低(P<0.05),骨髓T2值在USPIO注射前后差异具有显著统计学意义(P<0.05),T1DM组和对照组干骺端H型血管内皮细胞占比、平均荧光强度、T2值最大改变和最快时段速率、VV/TV、VN相较于骨干,差异均具有统计学意义(P<0.05)。结论基于USPIO增强MRI联合离体Micro-CT微血管成像定量可视化靶向评价T1DM兔骨髓H型血管结构是可行的,为探索糖尿病骨髓微血管病变提供影像学证据。Objective:To explore the feasibility of quantitative and visual targeting evaluation of type-H vessel structure in bone marrow of rabbits with type 1 diabetes mellitus(T1DM)based on ultra-small superparamagnetic nano-iron oxide(USPIO)enhanced MRI combined with in vitro micro-computed tomography(Micro-CT)microangiography.Materials and Methods:T1DM rabbits were constructed by injecting alloxan into the auricular vein in 20 out of 40 Japanese white rabbits(2-3 month-old).The remaining rabbits received equivalent volumes of normal saline via auricular marginal vein injection as controls.After 4 months of successful modeling,samples from metaphyseal and diaphyseal parts of tibia were taken respectively for sorting and identification of bone marrow Type-H vascular endothelial cells,Type-H vascular immunofluorescence detection,USPIO labeling of bone marrow endothelial cells in vitro and in vivo,MRI and Micro-CT microangiography of bone marrow in vitro.The proportion of Type-H vascular endothelial cells,average fluorescence intensity,T2 value of MRI,vessel volume/tissue volume(VV/TV)and vessel number(VN)were measured respectively.Results:Compared with the control group,the proportion of type-H vascular endothelial cells,average fluorescence intensity,VV/TV and VN of bone marrow were significantly decreased in T1DM rabbits(P<0.05),and the T2 value of MRI in bone marrow showed significant differences before and after USPIO injection(P<0.05).The metaphyseal and diaphysis of T1DM rabbits were respectively compared with the control group.The proportion of type-H vascular endothelial cells,average fluorescence intensity,maximum change of T2 value and fastest time rate,VV/TV and VN in metaphysis were significantly different from those in the diaphysis(P<0.05).Conclusions:USPIO enhanced MRI combined with in vitro Micro-CT microvascular imaging is feasible for quantitative and visual targeting evaluation of bone marrow type-H vascular structure in T1DM rabbits.This study provides imaging evidence for exploring diabetic bone marro

关 键 词：1型糖尿病兔 骨髓微血管 内皮细胞 超小超顺磁性纳米氧化铁 磁共振成像 显微CT 

分 类 号：R445.2[医药卫生—影像医学与核医学] R-332[医药卫生—诊断学]