伏马毒素B_(1)诱导棉铃虫HaCYP6AE19表达进而增强对高效氯氟氰菊酯的抗药性  

Fumonisin B_(1) induces the expression of HaCYP6AE19 in Helicoverpa armigera,thereby enhanceing resistance to λ-cyhalothrin

作　　者：郑小虎 赵倩倩 庞民好[1] 杨太新 刘颖超[1] 唐博文 ZHENG Xiao-Hu;ZHAO Qian-Qian;PANG Min-Hao;YANG Tai-Xin;LIU Ying-Chao;TANG Bo-Wen(College of Plant Protection,Hebei Agricultural University,Baoding 071000,China)

机构地区：[1]河北农业大学植物保护学院,保定071000

出　　处：《应用昆虫学报》2024年第6期1217-1229,共13页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金(32202299);河北省中央引导地方科技发展资金项目(236Z6504G);河北省青年拔尖人才项目(BJK2024007);河北农业大学引进人才科研专项(YJ2020012);国家现代农业产业技术体系项目(CARS-21)。

摘　　要：【目的】玉米耕作模式的转变使得伏马毒素B_(1)在玉米组织中积累,棉铃虫Helicoverpa armigera在取食玉米时便会摄入伏马毒素B_(1),但这对棉铃虫的抗药性有何影响未见报道。近年来,棉铃虫对高效氯氟氰菊酯的抗药性逐年升高,这是否与棉铃虫取食伏马毒素B_(1)有关尚不得知。本研究将以此为目的展开探讨,明确棉铃虫对高效氯氟氰菊酯抗药性升高的原因,为害虫抗药性产生提供新思路。【方法】在棉铃虫饲料中添加伏马毒素B_(1),测定了伏马毒素B_(1)对棉铃虫抗药性的影响,通过转录组测序和RNAi技术鉴定了关键解毒基因,利用RT-qPCR验证了伏马毒素B_(1)和高效氯氟氰菊酯对解毒基因表达量的影响。【结果】取食0.01和0.1 mg/kg伏马毒素B_(1)后,高效氯氟氰菊酯对棉铃虫的LC_(50)值分别为对照组的1.31和1.43倍;转录组测序鉴定到关键解毒基因HaCYP6AE19,在沉默HaCYP6AE19后,高效氯氟氰菊酯对棉铃虫的LC_(50)值为对照组的2.59倍;在伏马毒素B_(1)和高效氯氟氰菊酯胁迫下,1 d后,HaCYP6AE19表达量无明显变化(P>0.05),2 d后,表达量分别为对照组的4.44(P<0.01)和2.39倍(P<0.05),3 d后,表达量分别为对照组的5.27(P<0.01)和6.25倍(P<0.01)。【结论】摄入伏马毒素B_(1)后,棉铃虫解毒基因HaCYP6AE19过度表达,进而增强了棉铃虫对高效氯氟氰菊酯的抗药性。[Aim]To investigate whether the ingestion of fumonisin B_(1) in maize tissues by Helicoverpa armigera could contribute to the increase in resistance of this pest to λ-cyhalothrin.[Methods]Fumonisin B_(1) was added to the feed of H.armigera and its effect on the resistance of H.armigera to λ-cyhalothrin was determined.Key detoxification genes were identified by transcriptome sequencing and RNAi technology,and the effects of fumonisin B_(1) and λ-cyhalothrin on the expression of these genes was verified with RT-qPCR.[Results]After feeding on a diet containing 0.01 and 0.1 mg/kg of fumonisin B_(1),the LC_(50) values of H.armigera to λ-cyhalothrin were 1.31 and 1.43 times higher,respectively,than those of the control group.The key detoxification gene HaCYP6AE19 was identified by transcriptome sequencing.After silencing this gene,the LC_(50) value of H.armigera to λ-cyhalothrin was 2.59 times that of the control group.There was no significant change in HaCYP6AE19 expression after 1 day(P>0.05)of either fumonisin B_(1) or λ-cyhalothrin stress.However,after 2 days the expression of this gene in the fumonisin B_(1) and λ-cyhalothrin treatment groups was 4.44(P<0.01)and 2.39(P<0.05)times,respectively,that of the control,and after 3 days,its expression was 5.27(P<0.01)and 6.25(P<0.01)times,respectively,that of the control.[Conclusion]Ingestion of fumonisin B_(1) by H.armigera causes overexpression of the detoxification gene HaCYP6AE19 and thereby enhances resistance toλ-cyhalothrin.

关 键 词：棉铃虫 伏马毒素B_(1) HaCYP6AE19 高效氯氟氰菊酯 抗药性 

分 类 号：S435.622.3[农业科学—农业昆虫与害虫防治]