原弹性蛋白对胶原蛋白体外自组装和降解的生物力学影响  

Biomechanical Effects of Tropelastin on Collagen Self-Assembly and Degradation in vitro

作　　者：李军 张俊阳 张春秋[1,2] 王鑫[1,2] LI Jun;ZHANG Junyang;ZHANG Chunqiu;WANG Xin(Tianjin Key Laboratory for Advanced Mechatronic System Design and Intelligent Control,Tianjin University of Technology,Tianjin 300384,China;National Demonstration Center for Experimental Mechanical and Electrical engineering Education(Tianjin University of Technology),Tianjin 300384,China)

机构地区：[1]天津理工大学天津市先进机电系统设计与智能控制重点实验室,天津300384 [2]机电工程国家级实验教学示范中心(天津理工大学),天津300384

出　　处：《医用生物力学》2025年第1期210-215,共6页Journal of Medical Biomechanics

基　　金：天津市自然科学基金多元投入基金项目面上项目(21JCYBJC01210);南开大学眼科学研究院开放基金(NKYKD202207);天津理工大学2023年校级研究生科研创新实践项目(YJ2311)。

摘　　要：目的 研究原弹性蛋白对胶原蛋白体外自组装-降解的生物力学影响。方法 利用生物型原子力显微镜进行实时动态扫描,观察并分析胶原原纤维在自组装过程中的微观形貌、取向性、面积占比和平均直径变化;通过透射电子显微镜对胶原原纤维的D带结构进行表征;最后,借助Gwyddion和Matlab软件对数据进行分析,评估胶原原纤维的结构特性及其抗降解性能。结果 与对照组相比,原弹性蛋白的加入不仅促进了胶原蛋白的自组装,显著提高了自组装体所占的面积,还导致自组装体的方向性由单一方向性变为多向性。加入胶原酶后,自组装体会发生降解,但降解半周期随着原弹性蛋白的加入而减少,即原弹性蛋白的存在加速了胶原蛋白自组装体的降解。结论 原弹性蛋白的加入促进了胶原蛋白的自组装,增加了自组装体所占的面积,但自组装体的取向一致性有所减弱,降低了胶原原纤维抗酶降解的能力。研究结果为胶原基仿生功能材料的制备奠定了理论基础。Objective To investigate the biomechanical effects of tropoelastin on self-assembly and degradation of the collagen in vitro.Methods The real-time dynamic scanning with atomic force microscopy was utilized to observe and analyze the changes in microstructure,orientation,area fraction,and average diameter of collagen fibrils during the self-assembly process.The D-band structure of collagen fibrils was characterized using transmission electron microscopy.Finally,data analysis was performed using Gwyddion and Matlab software to assess the structural characteristics and degradation resistance of collagen fibrils.Results Compared to the control group,the addition of tropoelastin not only promoted the collagen self-assembly and significantly increased the area occupied by the assemblies,but also caused a shift in their orientation from unidirectional to multidirectional.Upon the addition of collagenase,the assemblies underwent degradation;however,the degradation half-period decreased with the inclusion of tropoelastin,indicating that tropoelastin accelerated the degradation of collagen assemblies.Conclusions The addition of tropoelastin promotes the collagen self-assembly,and increases the area occupied by the self-assembled structures.However,the consistency of orientation in the assemblies is weakened,resulting in a reduced resistance of collagen fibrils to degradation by collagenase.This study lays a theoretical foundation for the preparation of collagen-based biomimetic functional materials.

关 键 词：胶原蛋白 原弹性蛋白 自组装 胶原酶降解 生物力学 

分 类 号：R318.01[医药卫生—生物医学工程]