Inhibitory effects of Houttuynia cordata injection on hyphal growth and biofilm formation of Candida albicans  

作　　者：Qian-Wen Xu Ding-Hui Wang Cang-Cang Zhang Da-Qiang Wu Xiao-Jia Niu 

机构地区：[1]Department of Pathogenic Biology and Immunology,College of Integrated Chinese and Western Medicine,Anhui University of Chinese Medicine,Hefei 230012,China [2]Research Institute of Integrated Traditional Chinese and Western Medicine,Anhui Academy of Chinese Medicine,Hefei 230012,China [3]Key laboratory of Xin’an Medicine,Ministry of Education,Anhui University of Chinese Medicine,Hefei 230038,China

出　　处：《Infectious Diseases Research》2025年第1期28-34,共7页传染病研究(英文)

基　　金：sponsored by the projects funded by the Natural Science Foundation(Key project)of University in Anhui province(grant numbers 2023AH050727,2023AH040114,2023AH052278).

摘　　要：Background:Houttuynia cordata injection(HCI),made from fresh Houttuynia cordata,exerts heat-clearing,detoxifying and diuretic effects.It is indicated for various infections including lung abscess,fever,leucorrhea,urinary tract infection and carbuncle.Candida albicans is a common opportunistic pathogen in immunocompromised individuals.This pathogenic fungus colonizes in skin,mucosa membrane and digestive tract,potentially progressing from localized mucosal infections to systemic disease.Methods:The minimum inhibitory concentration(MIC)of HCI against C.albicans strain was determined by the microdilution method.The hyphal status of C.albicans was observed after incubation in the liquid medium and evaluated by Gram staining.The biofilm formation ability was measured using XTT reduction assay and assessed by Calcofluor White staining.The expression of virulence-related genes was detected with quantitative RT-PCR.Results:The MIC of HCI against C.albicans strain was determined to be 0.5 g/mL.At this concentration,HCI exhibited inhibitory effects on hyphal formation,as confirmed by both liquid medium observation and Gram staining.HCI at the MIC also effectively inhibited C.albicans biofilm formation,which was verified through XTT reduction assay and Calcofluor White staining.Additionally,gene expression analysis revealed that HCI significantly suppressed the expression of virulence-related genes in C.albicans.Conclusion:HCI demonstrates inhibitory effects on C.albicans growth and biofilm development.It inhibits hyphal formation,affecting the yeast-to-hyphal transition.This study investigated the antifungal effects of HCI,providing potent experimental evidence for its mechanism of action against C.albicans.

关 键 词：Houttuynia cordata injection Candida albicans MYCELIUM virulence factors biofilms 

分 类 号：R285.5[医药卫生—中药学]