血清25-(OH)D_(3)水平与儿童腹泻及免疫功能的相关性研究  

Correlation of serum 25-(OH)D_(3)level with diarrhea and immune function in children

作　　者：孙春涛 盛优静 杨霞 王雪娟 SUN Chuntao;SHENG Youjing;YANG Xia;WANG Xuejuan(Department of Clinical Laboratory,Ningxia Women and Children′s Hospital,Peking University First Hospital/Ningxia Hui Autonomous Region Maternal and Child Health Hospital,Yinchuan,Ningxia 750002,China)

机构地区：[1]北京大学第一医院宁夏妇女儿童医院/宁夏回族自治区妇幼保健院检验科,宁夏银川750002

出　　处：《检验医学与临床》2025年第5期587-590,共4页Laboratory Medicine and Clinic

基　　金：宁夏回族自治区卫生健康系统科研课题项目(2022-NWKY-035)。

摘　　要：目的探讨血清25-羟维生素D_(3)[25-(OH)D_(3)]水平与儿童腹泻及免疫功能的相关性。方法选取2021年11月至2023年11月在该院儿科门诊就诊的323例腹泻患儿作为病例组,另选取同期该院300例健康体检儿童作为对照组。检测并比较2组血清25-(OH)D_(3)、急性期炎症反应相关指标[白细胞计数(WBC)、C反应蛋白(CRP)、降钙素原(PCT)]、体液免疫指标[免疫球蛋白(IgA)、IgG、IgM)]、细胞免疫指标[CD4^(+)T细胞、CD8^(+)T细胞、CD19^(+)B细胞、CD16^(+)NK细胞/CD56^(+)NK细胞比值(CD16/CD16比值)]、炎症细胞因子[肿瘤坏死因子-α、干扰素(INF)-γ、白细胞介素(IL)-2、IL-4、IL-6、IL-10]水平的差异。采用Pearson相关分析腹泻患儿血清25-(OH)D_(3)水平与急性期炎症反应相关指标、炎症细胞因子、体液免疫指标、细胞免疫指标水平的相关性。结果病例组血清25-(OH)D_(3)、IgA、IgG、IgM、IL-4、IL-10水平及CD16/CD56比值均明显低于对照组,WBC、CRP、PCT、INF-γ、IL-2水平均明显高于对照组,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,腹泻患儿血清25-(OH)D_(3)水平与IgA、IgG、IgM、IL-4、IL-10水平及CD16/CD56比值均呈正相关(r=0.121、0.600、0.357、0.925、0.333、0.276,P<0.05),与WBC、CRP、PCT、INF-γ、IL-2水平均呈负相关(r=-0.359、-0.410、-0.610、-0.639、-0.286,P<0.05)。结论血清25-(OH)D_(3)水平降低可影响患儿的正常免疫功能,增加儿童腹泻发生率,检测血清25-(OH)D_(3)水平对儿童腹泻的诊治有重要意义。Objective To investigate the correlation of serum 25-hydroxyvitamin D_(3)[25-(OH)D_(3)]level with diarrhea and immune function in children.Methods A total of 323 children with diarrhea who visited the hospital from November 2021 to November 2023 were selected as the case group,anD_(3)00 healthy children who underwent physical examination during the same period were selected as the control group.The differences in the levels of serum 25-(OH)D_(3),acute inflammatory response related indicators[white blood cell count(WBC),C-reactive protein(CRP),procalcitonin(PCT)],humoral immune indicators[immunoglobulin(IgA),IgG,IgM],cellular immune indicators[the population of CD4^(+)T cell,CD8^(+)T cell,CD19^(+)B cell,CD16^(+)NK cell/CD56^(+)NK cell ratio(CD16/CD56 ratio)]and inflammatory cytokines(tumor necrosis factor-ɑ,interferon(INF)-γ,interleukin(IL)-2,IL-4,IL-6,IL-10)were detected and compared.Pearson correlation was used to analyze the correlation between serum 25-(OH)D_(3)level and the levels of inflammatory response related indicators,inflammatory cytokines,humoral immune indicators,and cellular immune indicators in the acute phase of inflammation in children with diarrhea.Results The levels of serum 25-(OH)D_(3),IgA,IgG,IgM,IL-4 and IL-10,CD16/CD56 ratio in the case group were significantly lower than those in the control group,and the levels of WBC,CRP,PCT,INF-γand IL-2 in the case group were significantly higher than those in the control group,and the differences were statistically significant(P<0.05).Pearson correlation analysis showed that the serum 25-(OH)D_(3)level in children with diarrhea was positively correlated with IgA,IgG,IgM,IL-4,IL-10 levels and CD161/CD56 ratio(r=0.121,0.600,0.357,0.925,0.333,0.276,P<0.05),and negatively correlated with WBC,CRP,PCT,INF-γand IL-2 levels(r=-0.359,-0.410,-0.610,-0.639,-0.286,P<0.05).Conclusion The decrease of serum 25-(OH)D_(3)level can affect the normal immune function of children and increase the incidence of diarrhea in children.Detection of serum 25-(OH)D_(3)level

关 键 词：儿童腹泻 25-羟维生素D_(3) 免疫球蛋白 细胞因子 炎症反应 

分 类 号：R725.7[医药卫生—儿科] R446.1[医药卫生—临床医学]