慢性心力衰竭患者血清miR-483-3p、miR-221-3p水平及其与心功能及MACE的关系  

Relationship between serum miR-483-3p and miR-221-3p levels and cardiac function and MACE in patients with chronic heart failure

作　　者：杨红霞 王月平 王国强 陈荣红 YANG Hongxia;WANG Yueping;WANG Guoqiang;CHEN Ronghong(Department of Cardiovascular,Inner Mongolia Baogang Hospital,Baotou,Inner Mongolia 014010,China)

机构地区：[1]内蒙古自治区包钢医院心血管内科,内蒙古包头014010

出　　处：《检验医学与临床》2025年第5期703-708,共6页Laboratory Medicine and Clinic

基　　金：内蒙古自治区自然科学基金项目(2020LH08021)。

摘　　要：目的探讨慢性心力衰竭患者血清微小RNA(miR)-483-3p、miR-221-3p水平及其与心功能及主要不良心血管事件(MACE)的关系。方法选取2022年1月至2023年12月该院收治的103例慢性心力衰竭患者作为观察组,根据是否发生MACE将其分为MACE组和非MACE组;另选取同期该院103例健康体检者作为对照组。采用实时荧光定量聚合酶链反应检测各组血清miR-483-3p、miR-221-3p水平。采用Pearson相关分析慢性心力衰竭患者血清miR-483-3p、miR-221-3p水平与心功能指标水平的相关性。采用受试者工作特征(ROC)曲线分析miR-483-3p、miR-221-3p对慢性心力衰竭患者发生MACE的预测价值。采用逐步向前法筛选变量,以多因素Logistic回归分析慢性心力衰竭患者发生MACE的影响因素。结果观察组血清miR-483-3p、miR-221-3p水平及左心房内径(LA)、左心室舒张末期内径(LEVDD)均高于对照组,左室射血分数(LVEF)、左室短轴缩短率(FS)均低于对照组,差异均有统计学意义(P<0.05)。MACE组LVEF、FS、LA、LEVDD,以及总胆固醇、miR-483-3p、miR-221-3p水平与非MACE组比较,差异均有统计学意义(P<0.05)。Pearson相关分析结果显示,慢性心力衰竭患者血清miR-483-3p、miR-221-3p水平与LA、LEVDD均呈正相关(P<0.05),与LVEF、FS均呈负相关(P<0.05)。ROC曲线分析结果显示,血清miR-483-3p、miR-221-3p联合检测预测慢性心力衰竭患者发生MACE的曲线下面积为0.856,明显大于二者单独检测的0.767、0.758,差异均有统计学意义(Z=2.068、2.195,P=0.039、0.028)。多因素Logistic回归分析结果显示,LVEF、FS、miR-483-3p和miR-221-3p均为慢性心力衰竭患者发生MACE的影响因素。结论慢性心力衰竭患者血清miR-483-3p、miR-221-3p水平均升高,二者与患者心功能及MACE紧密相关。Objective To investigate the relationship between serum microRNA(miR)-483-3p and miR-221-3p levels and cardiac function and major adverse cardiovascular events(MACE)in patients with chronic heart failure.Methods A total of 103 patients with chronic heart failure admitted to the hospital from January 2022 to December 2023 were selected as the observation group,and they were divided into MACE group and non-MACE group according to whether MACE occurred.At the same time,103 healthy people were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction was used to detect the levels of serum miR-483-3p and miR-221-3p in each group.Pearson correlation analysis was used to analyze the correlation between serum miR-483-3p,miR-221-3p and cardiac function index levels in chronic heart failure patients.Receiver operating characteristic(ROC)curve was used to analyse the predictive value of miR-483-3p and miR-221-3p levels for MACE in patients with chronic heart failure.Multivariate Logistic regression was used to analyze the influencing factors of MACE in patients with chronic heart failure.Results The levels of serum miR-483-3p and miR-221-3p,left atrial diameter(LA)and left ventricular end-diastolic diameter(LEVDD)in the observation group were higher than those in the control group,and the left ventricular ejection fraction(LVEF)and left ventricular fractional shortening(FS)were lower than those in the control group,and the differences were statistically significant(P<0.05).There were significant differences in LVEF,FS,LA,LEVDD,total cholesterol,miR-483-3p and miR-221-3p levels between the MACE group and the non-MACE group(P<0.05).Pearson correlation analysis showed that the levels of serum miR-483-3p and miR-221-3p in chronic heart failure patients were positively correlated with LA and LEVDD(P<0.05),and negatively correlated with LVEF and FS(P<0.05).The results of ROC curve analysis showed that the area under the curve of the combined detection of serum miR-483-3p and miR-221-3p to predict

关 键 词：慢性心力衰竭 微小RNA-483-3p 微小RNA-221-3p 心功能 主要不良心血管事件 

分 类 号：R541.6[医药卫生—心血管疾病] R446.1[医药卫生—内科学]