黄曲霉毒素B_(1)荧光微球偶联抗体工艺优化  

Optimization of antibody coupling processes for Aflatoxin B_(1) fluorescent microspheres

作　　者：谷瑞丽 苗银萍 赵林萍 曾小宇 瞿超杰 李宁 GU Rui-li;MIAO Yin-ping;ZHAO Lin-ping;ZENG Xiao-yu;QU Chao-jie;LI Ning(Key Laboratory of Food Safety Quick Testing and Smart Supervision Technology for State Market Regulation,Zhengzhou 450000,China;Henan Institute of Food and Salt Industry Inspection Technology,Zhengzhou 450000,China;Zhengzhou Zhongdao Biotechnology Co.,Ltd.,Zhengzhou 450000,China)

机构地区：[1]国家市场监管重点实验室(食品安全快速检测与智慧监管技术),河南郑州450000 [2]河南省食品和盐业检验技术研究院,河南郑州450000 [3]郑州中道生物技术有限公司,河南郑州450000

出　　处：《粮食与饲料工业》2025年第1期122-126,共5页Cereal & Feed Industry

摘　　要：为了保证黄曲霉毒素B_(1)(AFB_(1))时间分辨荧光免疫层析试纸条检测结果的灵敏度、均一性,对微球偶联抗体工艺进行了研究。通过一系列优化实验,确定荧光免疫层析试纸条微球偶联抗体工艺,为试纸条制备提供了重要的中间物。实验结果表明,研究建立的高灵敏荧光微球偶联方法,有效提高了荧光免疫层析试纸条的抗体偶联效率。To ensure the sensitivity and uniformity of detection results for Aflatoxin B_(1)(AFB_(1))time-resolved fluorescence immunochromatographic test strips,this study optimized the process for antibody coupling with fluorescent microspheres.A series of experiments were conducted to establish an optimal coupling method,providing critical intermediate materials for test strip production.Results show that the developed highsensitivity fluorescent microsphere coupling method significantly enhances the antibody coupling efficiency of the test strips.

关 键 词：AFB_(1) 免疫层析技术 荧光微球 偶联 

分 类 号：TS210.7[轻工技术与工程—粮食、油脂及植物蛋白工程]