PM_(2.5)中重金属砷呼吸暴露对肠道菌群结构及代谢的影响  

The effects of long-term respiratory exposure to heavy metal arsenic in PM_(2.5) on the structure and metabolism of gut microbiota

作　　者：张敏娟 余梓勋 易运红 田苗 周银锋 冯杰 祁增华 ZHANG Minjuan;YU Zixun;YI Yunhong;TIAN Miao;ZHOU Yinfeng;FENG Jie;QI Zenghua(Public Course Teaching Department,Henan Geology Mineral College,Zhengzhou,451464,China;School of Environmental Science and Engineering,Guangdong University of Technology,Guangzhou,510006,China;School of Chemistry and Chemical Engineering,Guangdong Pharmaceutical University,Zhongshan,528458,China)

机构地区：[1]河南地矿职业学院公共课教学部,郑州451464 [2]广东工业大学环境科学与工程学院,广州510006 [3]广东药科大学医药化工学院,中山528458

出　　处：《环境化学》2025年第2期432-441,共10页Environmental Chemistry

基　　金：国家自然科学基金(21806025);国家重点研发计划项目(2019YFC1804604)资助.

摘　　要：重金属砷(As)不仅具有致癌性,还能诱发和加剧心血管、呼吸以及生殖等多种系统疾病.有色金属冶炼区域大气细颗粒物(PM_(2.5))载带的重金属As(PM_(2.5)-As)具有浓度高、传播广、危害大以及生物吸收效率高等特点,因此,能够对机体造成更大程度的损伤和危害.现有的研究表明肠道微生物是环境污染物暴露诱发机体损伤的重要“枢纽”.然而,关于有色金属冶炼场地PM_(2.5)-As吸入暴露对肠道菌微生物群落多样性及其代谢功能影响的研究仍存在不足.本研究依据有色金属冶炼场地PM_(2.5)-As的环境浓度及组成开展动物暴露研究,探究PM_(2.5)-As暴露对肠道菌群组成及代谢的影响.结果显示,PM_(2.5)-As暴露显著影响了微生物菌群的丰度和特征结构,其中乳酸杆菌是受影响最大的肠道菌属.在此基础上,使用非靶向代谢组学方法,共筛选出317种差异代谢物,而色氨酸代谢是受PM_(2.5)-As暴露影响最为显著的代谢通路.本研究不仅为PM_(2.5)-As的健康危害提供了新的证据,还为相关疾病的预防和治疗提供了科学依据.Heavy metal arsenic(As)not only has carcinogenicity,but also can induce and aggravate cardiovascular,respiratory,reproductive and other systemic diseases.The heavy metal As carried by atmospheric fine particles(PM_(2.5)-As)in non-ferrous metal smelting areas has the characteristics of high concentration,widespread,great harm and high biological absorption efficiency.Therefore,it can cause greater damage and harm to the body.Previous studies have shown that gut microbiota was an important“hub”for body damage induced by environmental pollutant exposure.However,the effects of PM_(2.5)-As inhalation exposure on intestinal microbial community diversity and metabolism in non-ferrous metal smelting sites remain to be explored.In this study,animal exposure studies were carried out based on the environmental concentration and composition of PM_(2.5)-As in non-ferrous metal smelting sites to explore the effects of PM_(2.5)-As exposure on the composition and metabolism of gut microbiota.The results showed that PM_(2.5)-As exposure significantly affected the abundance and characteristic structure of gut microbiota,among which Lactobacillus was the most affected gut microbiota.On this basis,a total of 317 metabolites were screened by non-targeted metabolomics,and tryptophan metabolism was the most significant metabolic pathway affected by PM_(2.5)-As exposure.The results of this paper not only provide new evidence for the health hazards of PM_(2.5)-As,but also provide the scientific basis for the prevention and treatment of related diseases.

关 键 词：PM_(2.5)-As 吸入暴露 肠道菌群 代谢通路 

分 类 号：X-1[环境科学与工程] O6[理学—化学]