AuNRs-PDMS微柱结构的制备及对被调控活细胞的无标记SERS检测  

Fabrication of AuNRs-PDMS Micropillar Structure and Its Label-free SERS Detection for Regulated Living Cells

作　　者：吴晓宇 朱峰 况源桁 刘伯华 孙崇玲[1] 王艳艳 WU Xiaoyu;ZHU Feng;KUANG Yuanheng;LIU Bohua;SUN Chongling*;WANG Yanyan*(State Key Laboratory of Precision Measurement Technology and Instruments,College of Precision Instruments and Optoelectronics Engineering,Tianjin University,Tianjin 300072,China)

机构地区：[1]精密测试技术及仪器全国重点实验室,天津大学精密仪器与光电子工程学院,天津300072

出　　处：《高等学校化学学报》2025年第3期25-33,共9页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金(批准号:32371471)资助。

摘　　要：设计制备了金纳米棒-聚二甲基硅氧烷(AuNRs-PDMS)微柱结构,利用微柱结构的地形特征作为机械线索进行细胞调控,同时在微柱结构上组装AuNRs作为表面增强拉曼散射(SERS)基底,用于被调控细胞的无标记SERS检测.首先对比分析了利用溶剂蒸发法、吸附转移法和压印转移法3种AuNRs组装及修饰方法获得的AuNRs-PDMS微柱结构的SERS增强性能和结构均一性.通过比较不同测试点处4-巯基苯甲酸(4-MBA)的拉曼光谱信号变化发现,由压印转移法获得的AuNRs-PDMS微柱结构具有较高的SERS增强性能,同时也可保证基底的良好均一性,相对标准偏差(RSD)为7.3%,表明该结构可以作为细胞无标记SERS检测的基底.以间充质干细胞(MSCs)作为调控与检测对象,将其分别培养于由压印转移法获得的AuNRs-PDMS微柱结构以及AuNRs-PDMS平面结构上,发现受到微柱调控作用的细胞呈现出沿地形特征生长的特点,并且细胞形貌发生了改变.同时,对细胞的无标记SERS检测结果表明,被调控细胞的脂质含量增多,蛋白质含量和分子键的拉伸振动模式也发生了改变,这暗示了被调控细胞的接触导向和形貌变化与其脂质和蛋白质含量变化相关,进一步揭示了细胞-基底的相互作用关系.In this work,gold nanorods-polydimethylsiloxane(AuNRs-PDMS)micropillar was proposed,of which topographic feature could serve as mechanical cues for cell regulation,as well as AuNRs assembly on the micropillar could serve as surface-enhanced Raman scattering(SERS)substrates for regulated cell detection.Three fabrication routes,including solvent evaporation,absorption transferring and stamp transferring,were compared and optimized towards the construction of AuNRs-PDMS micropillar with high SERS enhancement and homogeneity.The SERS performance was determined by comparing the spectra changes of 4-mercaptobenzoic acid(4-MBA)at different test points on AuNRs-PDMS micropillar.Results demonstrate that AuNRs-PDMS micropillar by stamp transferring possesses acceptable SERS enhancement with the highest homogeneity,and the relative standard deviation(RSD)of SERS intensity is 7.3%,indicating the structure is a feasible SERS substrate for label-free cell detection.Mesenchymal stem cells(MSCs),the object for cell regulation and SERS detection,were cultured on the AuNRs-PDMS micropillar by stamp transferring and AuNRs-PDMS flat control.Compared to their counterparts,MSCs regulated by the AuNRs-PDMS micropillar show growth direction along the topographic feature and morphology changes.Meanwhile,the variation of SERS spectra illustrates the increase of lipids and the changes of protein components as well as bond stretching vibrations during cell regulation,implying that the contact guidance and morphology changes of regulated cells are associated with cell component variations.The strategy builds a bridge between cell regulation and label-free SERS detection of the regulated cells,providing deeper insight into cell-substrate interactions.

关 键 词：金纳米棒 表面增强拉曼光谱 细胞检测 细胞调控 

分 类 号：O657.37[理学—分析化学]