MEC/LEC-RI法对不同产地酒制多花黄精中糖类成分的定性与定量  

Qualitative and Quantitative Analysis on Saccharide Components in Polygonatum cyrtonema from Different Producing Areas by LEC-MEC-RI

作　　者：罗雨欣 刘嘉钰 李思梦 陈光宇[1] 谢梦洲[1] LUO Yu-xing;LIU Jia-yu;LI Si-meng;CHEN Guang-yu;XIE Meng-zhou(Hunan University of Chinese Medicine/Engineering Technology Research Center for Medicinal and Functional Food,Hunan University of Chinese Medicine/Key Laboratory of TCM Heart and Lung Syndrome Differentiation&Medicated Diet and Dietotherapy,Hunan University of Chinese Medicine/Provincial Key Laboratory of TCM Diagnostics,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学/湖南中医药大学药食同源功能性食品工程技术研究中心/湖南中医药大学中医心肺病证辨证与药膳食疗重点研究室/湖南中医药大学中医诊断学湖南省重点实验室,长沙410208

出　　处：《中国食物与营养》2025年第2期37-42,共6页Food and Nutrition in China

基　　金：湖南省重点研发计划(2021-2022年)项目(第二批)(项目编号:2022SK2018);2023年度湖南省中医药科研课题(项目编号:B2023134)。

摘　　要：目的:建立不同产地酒制多花黄精中糖类成分的定性与定量方法。方法:以11个产地的酒制多花黄精为研究样品,采用分子排阻/配体交换色谱-示差折光检测法(MEC/LEC-RI)分析酒制多花黄精糖类成分分子量,构建MEC-RI指纹图谱,测定D-葡萄糖、D-果糖含量,采用硫酸-蒽酮-紫外光谱法测定酒制多花黄精中黄精多糖含量,对糖类成分定性与定量结果进行关联性分析。结果:酒制多花黄精水浸液中主要含有分子量低于1 000 Da的低聚多糖,从MEC-RI指纹图谱中指认了5个共有峰,分子量分别约为220k、160k、100k、14.5k、0.95k Da,11个产地样品相似度均大于0.9。酒制多花黄精中D-葡萄糖、D-果糖含量在58.771~116.684、143.087~162.054 mg/g范围之间,黄精多糖含量在42.769~76.065 mg/g范围之间。结论:建立了不同产地酒制多花黄精中糖类成分的定性与定量方法,比较了不同产地酒制多花黄精中糖类成分的差异,可为多花黄精质量标准研究、资源分布及产品开发提供科学依据。【Objective】To establish the qualitative and quantitative methods of saccharides in the Polygonatum cyrtonema from differ-ent producing areas.【Method】Polygonatum cyrtonema from 11 producing areas as the research sample,the molecular weight of sac-charides was analyzed by Molecular Exclusion Chromatography-Differential Refraction(MEC-RI),the MEC-RI fingerprint chromatography was constructed by MEC-RI,the content of D-glucose,D-fructose was measured by Ligand Exchange Chromatography-Differential Refraction(LEC-RI),and the content of polysaccharide was measured by Sulfuric Acid-Phenol-Ultraviolet Spectrum(UV),correlation analysis was carried out between qualitative and quantitative results of carbohydrate components.【Result】The oligopolysac-charide with molecular weight lower than 1000Da were mainly contained in the aqueous extract of Polygonatum cyrtonema,the molecular weights of 5 common peaks were about 220k,160k,100k,14.5k and 0.95k Da respectively were identified from MEC-RI fingerprints,the similarity of samples from 11 producing areas was all greater than 0.9,the contents of D-glucose and D-fructose in Polygonatum cyrtonema were between 58.771—116.684,143.087—162.054 mg/g,the total polysaccharide content was in the range of 42.769—76.065 mg/g.【Conclusion】The qualitative and quantitative methods of polysaccharide in Polygonatum cyrtonema from different producing areas were established,and the differences of polysaccharide in Polygonatum cyrtonema from different areas were compared,which can provide scientific basis for quality standard research,resource distribution and product development of Polygonatum cyrtonema.

关 键 词：多花黄精 配体交换色谱 分子排阻色谱 示差折光 多糖 

分 类 号：O657[理学—分析化学] R284.1[理学—化学]