树莓粗多糖对UVB诱导的小鼠皮肤光损伤的防护作用  

Protective effects of raspberry crude polysaccharides against UVB-induced skin photodamage in BALB/c mice

作　　者：王雨婷 王学红 陆杰 米尔扎提·阿不力米提 车广学 韩乐 姬一鸣 杨永晶 WANG Yuting;WANG Xuehong;LU Jie;Mirzat Alimet;CHE Guangxue;HAN Le;JI Yiming;YANG Yongjing(College of Ecology and Environment Engineering,Qinghai University,Xining,Qinghai 810016,China)

机构地区：[1]青海大学生态环境工程学院,青海西宁810016

出　　处：《湖南农业大学学报(自然科学版)》2025年第1期107-112,共6页Journal of Hunan Agricultural University(Natural Sciences)

基　　金：“西部之光”人才培养计划项目(2023—2025);青海大学生态环境工程学院生态学世界一流学科研究生科技创新项目(2023-stxy-Y18)。

摘　　要：采用树莓粗多糖(RCP)(剂量分别为50、100、200mg/kg)对BALB/c小鼠中波紫外线(UVB)皮肤光损伤模型进行处理,采用酶联免疫吸附法(ELISA)和微板法测定小鼠皮肤组织中基质金属蛋白酶(MMPs)、炎症因子、氧化应激等相关生理生化指标,研究树莓粗多糖对UVB皮肤光损伤的防护作用。结果表明,RCP可明显改善BALB/c小鼠皮肤红斑、脱屑和皱纹,并减轻皮肤局部角化过度、表皮增厚、纤维组织增生及淋巴细胞浸润。相较于模型组,低剂量RCP组肿瘤坏死因子-α(TNF-α)水平降低了16.1%,同时基质金属蛋白酶-1(MMP-1)与基质金属蛋白酶-3(MMP-3)的含量分别减少了22.1%与43.3%;中剂量RCP组丙二醛(MDA)含量下降18.9%,TNF-α水平降低37.4%,MMP-1和MMP-3含量分别减少35%和48.2%;而在高剂量RCP组中,超氧化物歧化酶(SOD)与过氧化氢酶(CAT)的活性分别增强了21.3%和34.3%,MDA含量下降了24.8%,白细胞介素-6(IL-6)与TNF-α水平分别降低了33.3%和56.6%,此外,MMP-1和MMP-3的含量也分别减少了35%和63.3%。以上结果说明RCP可通过降低基质金属蛋白酶含量、减轻炎症反应及缓解氧化应激来改善UVB引起的小鼠皮肤光损伤,且RCP高剂量组的效果最佳。To investigate the protective effect of raspberry crude polysaccharides(RCP)against ultraviolet B(UVB)-induced skin photodamage in BALB/c mice,RCP was administered to UVB-induced BALB/c mice skin photodamage model at doses of 50,100 and 200 mg/kg and the levels of matrix metalloproteinases,inflammatory factors and oxidative stress-related indices in skin tissue were measured by enzyme-linked immunosorbent assay(ELISA)or microplate assay.The results showed RCP significantly alleviated erythema,desquamation,wrinkles,local hyperkeratosis,epidermal thickening,fibrous tissue hyperplasia and lymphocytic infiltrate of the skin damaged by UVB.Compared to the model group,in the low-dose group,there was a 16.1%reduction in tumor necrosis factor-α(TNF-α)levels,accompanied by a decrease of 22.1%and 43.3%in matrix metalloproteinase-1(MMP-1)and matrix metalloproteinase-3(MMP-3)content,respectively.In the medium-dose group,malondialdehyde(MDA)content decreased by 18.9%,TNF-αlevels dropped by 37.4%,and MMP-1 and MMP-3 content decreased by 35%and 48.2%,respectively.For the high-dose group,superoxide dismutase(SOD)and catalase(CAT)activities were enhanced by 21.3%and 34.3%,respectively,while MDA content decreased by 24.8%.Interleukin-6(IL-6)and TNF-αlevels were reduced by 33.3%and 56.6%,respectively.Additionally,MMP-1 and MMP-3 content decreased by 35%and 63.3%,respectively.The above results indicated that RCP could ameliorate UVB-induced skin photodamage by inhibiting matrix metalloproteinases content,attenuating inflammation response and oxidative stress in BALB/c mice,and the high dose of RCP showed the best effect.

关 键 词：树莓粗多糖 小鼠皮肤光损伤 中波紫外线 基质金属蛋白酶 炎症因子 氧化应激 

分 类 号：R285.5[医药卫生—中药学]