聚乙烯吡咯烷酮抑制蒙古栎组培褐化效果及其生理响应  

Inhibiting Effect of PVP on Tissue Browning of Quercus mongolica and Its Physiology Response During Culture

作　　者：杨悦[1] 吴晓微 董思贤 沈海龙[1] 杨玲[1,2] YANG Yue;WU Xiaowei;DONG Sixian;SHEN Hailong;YANG Ling(State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,China;The College of Forestry,Beijing Forestry University,Beijing 100091,China)

机构地区：[1]林木遗传育种全国重点实验室(东北林业大学),哈尔滨150040 [2]北京林业大学林学院,北京100091

出　　处：《森林工程》2025年第2期288-297,共10页Forest Engineering

基　　金：国家重点研发计划(2023YFD2200103);中央高校基本科研业务费专项资金项目(2572023CT02)。

摘　　要：为降低蒙古栎(Quercus mongolica)组培过程褐化现象,阐明褐化发生是否与抗氧化酶系统及酚类物质产生有关。以蒙古栎成熟合子胚为材料,利用组织培养技术优化蒙古栎再生植株体系获得再生植株,探究聚乙烯吡咯烷酮(PVP)抑制褐化处理对蒙古栎合子胚萌发的生理生化指标的影响。结果表明,PVP 40(40代表PVP平均分子量范围)抑制褐化效果最佳,添加0.2 g/L PVP 40不定芽诱导率最高,为71.17%,其芽增殖系数为3.90,芽生根率为40.37%,移栽存活率为73%;PVP降低了蒙古栎外植体抗氧化酶、多酚氧化酶活性及总酚酶活力,有效抑制褐化现象。PVP有效降低蒙古栎组培褐化效果抑制褐化不再加剧,优化蒙古栎器官再生植株体系,分析PVP抑制褐化处理对蒙古栎合子胚萌发的内在生理机制,为栎属其他树种抑制褐化提供参考。In order to reduce the browning phenomenon of Quercus mongolica during tissue culture,it was clarified whether the browning was related to the production of antioxidant enzyme system and phenolic substances.Mature zygotic embryos of Q.mongolica were used as materials,and the regeneration plant system of Q.mongolica was optimized by tissue culture technology to obtain regenerated plants.The effects of PVP on the physiological and biochemical indexes of zygotic embryo germination of Q.mongolica were investigated.The results indicated that PVP 40 had the best effect on inhibiting browning(40 represents the average molecular weight range of PVP).The highest induction rate of adventitious buds was 71.17%when 0.2 g/L PVP 40 was added.The bud proliferation coefficient was 3.90,the rooting rate was 40.37%,and the transplanting survival rate was 73%.PVP reduced the activity of antioxidant enzymes,polyphenol oxidase and total phenolase activity of Quercus mongolica explants,and effectively inhibited browning.This study effectively reduced the browning effect of tissue culture of Q.mongolica and no longer aggravated the browning inhibition,optimized the organ regeneration plant system of Q.mongolica,and analyzed the internal physiological mechanism of PVP inhibition browning treatment on the germination of zygotic embryos of Q.mongolica,so as to provide reference for other species of Q.mongolica to inhibit browning.

关 键 词：蒙古栎 聚乙烯吡咯烷酮 褐化 植株再生 生理生化 

分 类 号：S792.186[农业科学—林木遗传育种]