电针对溃疡性结肠炎模型小鼠IL-4/STAT6/SPDEF通路相关蛋白表达的影响  

Effects of Electroacupuncture on IL-4/STAT6/SPDEF Pathway-Related Protein Expressions in Ulcerative Colitis Model Mice

作　　者：胡晓妹[1] 易细芹 展立芬 李芊 丁强盛 邓石峰 张泓[1] HU Xiaomei;YI Xiqin;ZHAN Lifen;LI Qian;DING Qiangsheng;DENG Shifeng;ZHANG Hong(College of Acupuncture,Moxibustion and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410007,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,湖南长沙410007

出　　处：《中国中医药信息杂志》2025年第3期112-118,共7页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：湖南省自然科学基金青年科学基金(2022JJ40316);湖南省研究生科研创新重点项目(X20210682);湖南中医药大学中西医结合一流学科项目(2020ZXYJH37)。

摘　　要：目的观察电针对溃疡性结肠炎(UC)模型小鼠IL-4/STAT6/SPDEF通路相关蛋白表达的影响,探讨其改善肠黏液屏障的作用机制。方法48只BALB/c小鼠随机分为空白组12只和造模组36只,造模组采用3%葡聚糖硫酸钠自由饮用建立UC模型,造模成功后随机分为模型组、电针组、柳氮磺吡啶(SASP)组,每组12只。电针组针刺“关元”“天枢”“足三里”“上巨虚”,每日20 min,SASP组每日予SASP混悬液(500 mg/kg)灌胃,空白组不干预,模型组仅捆绑,连续7 d。观察小鼠一般状况并进行疾病活动指数(DAI)评分,HE染色观察结肠组织形态,进行结肠黏膜损伤指数(CMDI)评分,AB-PAS染色观察结肠组织杯状细胞数量,免疫荧光染色检测结肠组织黏蛋白2(MUC2)蛋白表达,ELISA测定血清肿瘤坏死因子-α(TNF-α)、γ干扰素(IFN-γ)含量,Western blot检测结肠组织白细胞介素(IL)-4、信号转导与转录激活因子(STAT)6、上皮特异性ETs转录因子(SPDEF)蛋白表达。结果与空白组比较,模型组小鼠出现黏液便、便血,饮食饮水量减少,体质量下降,DAI、CMDI评分明显升高(P<0.01),肠绒毛脱落坏死,结肠隐窝-绒毛结构破坏,伴有严重中性粒细胞浸润,黏液层变薄,杯状细胞萎缩、数量减少(P<0.01),结肠组织MUC2表达明显降低(P<0.01),血清TNF-α、INF-γ含量明显升高(P<0.01),结肠组织IL-4、STAT6、SPDEF蛋白表达明显降低(P<0.01);与模型组比较,电针组与SASP组小鼠黏液便、便血减轻,体质量升高,DAI、CMDI评分明显降低(P<0.05),肠上皮形态基本正常,腺体结构改善,少量炎性细胞浸润和轻微水肿,黏液层厚度有所恢复,杯状细胞萎缩减轻、数量增加(P<0.05),结肠组织MUC2表达明显升高(P<0.05),血清TNF-α、IFN-γ含量明显降低(P<0.05),结肠组织IL-4、STAT6、SPDEF蛋白表达明显升高(P<0.05)。结论电针可促进UC模型小鼠肠黏液屏障修复,其机制可能与激活IL-4/STAT6/SPDEF通路相关蛋白表达有关。Objective To observe the effects of electroacupuncture on IL-4/STAT6/SPDEF pathway-related protein expressions in model mice of ulcerative colitis(UC);To explore the mechanism of electroacupuncture improving the intestinal mucus barrier.Methods Totally 48 BALB/c mice were randomly divided into blank group(12 mice)and modeling group(36 mice).The UC models were established in the modeling group using 3%dextran sulfate sodium free drinking.After successful modeling,mice were further randomly divided into model group,electroacupuncture group and sulfasalazine(SASP)group,with 12 mice in each group.The electroacupuncture group received electroacupuncture at“Guanyuan”,“Tianshu”,“Zusanli”and“Shangjuxu”acupoints for 20 minutes per day,the SASP group was administered sulfasalazine enteric-coated tablet suspension(500 mg/kg)by gavage,the blank group received no intervention,the model group was only confined for continuous 7 days.The general conditions were observed and disease activity index(DAI)score were recorded,HE staining was used to observe the morphology of colonic tissue and evaluate the colonic mucosal damage index(CMDI)score,AB-PAS staining was used to observe the number of goblet cells in colonic tissue,and immunofluorescence staining was used to detect the expression of mucin 2(MUC2)protein in colonic tissue,ELISA was used to detect the contents of serum tumor necrosis factorα(TNF-α)andγinterferon(IFN-γ),and Western blot was used to detect the protein expressions of interleukin(IL)-4,signal transducer and activator of transcription(STAT)6 and epithelial specific transcription factor(SPDEF)in colonic tissue.Results Compared with the blank group,the model group showed mucous stool,bloody stool,reduced intake of food and water,reduced body mass,increased DAI and CMDI scores(P<0.01),with intestinal villi shedding and necrosis,destruction of colonic crypt villi structure,accompanied by severe neutrophil infiltration,thinning of mucus layer,atrophy and reduction of goblet cells(P<0.01),the expressi

关 键 词：溃疡性结肠炎 电针 黏液屏障 IL-4/STAT6/SPDEF通路 杯状细胞 小鼠 

分 类 号：R245.3[医药卫生—针灸推拿学]