丁香酚对合果芋果胶杆菌(Pectobacterium aroidearum)L6抑菌效果研究  

Antibacterial Effect of Eugenol on Pectobacterium aroidearum L6

作　　者：禄锦鹏 刘文波 范鸿雁[2] 缪卫国 林春花 秦春秀 LU Jin-peng;LIU Wen-bo;FAN Hong-yan(Tropical Agriculture and Forestry Colloege,Hainan University/Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests,Ministry of Education,Haikou,Hainan 570228;Institution of Tropical Fruit Trees,Hainan Academy of Agricultural Sciences,Haikou,Hainan 571199)

机构地区：[1]海南大学热带农林学院/热带农林生物灾害绿色防控教育部重点实验室,海南海口570228 [2]海南省农业科学院热带果树研究所,海南海口571199

出　　处：《安徽农业科学》2025年第4期124-128,共5页Journal of Anhui Agricultural Sciences

基　　金：海南省自然科学基金项目(322MS025,321RC470);海南省院士创新平台科研专项(YSPTZX202018)。

摘　　要：以实验室前期分离的果胶杆菌(P.aroidearum)L6为对象,探究丁香酚对其抑菌效果。通过常量稀释法测定丁香酚对合果芋果胶杆菌(P.aroidearum)L6的抑菌活性;利用紫外分光光度法测定经丁香酚处理后菌液的K^(+)、AKP、ATP、核酸和蛋白质含量;测定其电导率,再利用扫描电子显微镜观察经丁香酚处理后的菌体,从细胞水平探究丁香酚抑菌效果。结果显示,丁香酚对合果芋果胶杆菌(P.aroidearum)L6的MIC为800 mg/L,EC_(50)为409.33 mg/L;通过对比丁香酚处理前后的菌体破坏特征,发现K^(+)、AKP、核酸、蛋白质和电导率上升,ATP水平下降;扫描电子显微镜观察显示,丁香酚处理的菌体出现裂解、黏连现象。丁香酚通过破坏果胶杆菌(P.aroidearum)L6细胞膜完整性,引起内含物泄露、能量代谢失衡,从而达到抑菌作用。The study investigated the antibacterial effect of eugenol against P.aroidearum L6,which was isolated in the laboratory.The antibacterial activity of eugenol was determined using the constant dilution method.Ultraviolet spectrophotometry was used to measure the levels of K~+,AKP,ATP,nucleic acid,and protein in the bacterial solution treated with eugenol.The electric conductivity was measured.Scanning electron microscopy was used to observe eugenol-treated bacteria and explore the antibacterial effect at the cellular level.The MIC of eugenol for P.aroidearum L6 was 800 mg/L.And the EC_(50)of eugenol for P.aroidearum L6 was 409.33 mg/L.Comparison of bacterial damage characteristics before and after eugenol treatment revealed increased levels of K~+,AKP,nucleic acid,protein,electric conductivity and decreased ATP levels.Scanning electron microscopy images showed lysed and adhered bacteria following eugenol treatment.Eugenol disrupts the integrity of the cell membrane of P.aroidearum L6,causing leakage of intracellular contents and an imbalance in energy metabolism,thereby achieving its antibacterial effect.

关 键 词：丁香酚 果胶杆菌 抑菌作用 细胞膜 

分 类 号：S482.2[农业科学—农药学]