粉红螺旋聚孢霉微胶囊的制备及其对南方根结线虫病的防治效果  

Preparation of Microcapsule Containing Clonostachys rosea and Its Control Efficiency against Meloidogyne incognita

作　　者：张洁[1] 宋雅静 夏明聪[1] 耿书宝 孙润红[1] 徐文 张婧桦 武超[1] 杨丽荣[1] ZHANG Jie;SONG YaJing;XIA Mingcong;Geng Shubao;SUN Runhong;XU Wen;ZHANG Jinghua;WU chao;YANG Lirong(Henan Biopesticide Engineering Research Center/Henan International Joint Laboratory of Crop Protection/Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;School of Agronomy,Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]河南省农业科学院植物保护研究所/河南省生物农药工程研究中心/河南省作物保护国际联合实验室,郑州450002 [2]信阳农林学院农学院,信阳464000

出　　处：《中国生物防治学报》2025年第1期103-111,共9页Chinese Journal of Biological Control

基　　金：河南省科技研发计划联合基金(应用攻关类)项目(232103810015);河南省农业科学院基础性科研工作项目(2024JC08);河南省农业科学院自主创新项目(2024ZC067)。

摘　　要：随着设施蔬菜产业的快速发展,南方根结线虫病的发生和危害日趋严重,生物防治是一种安全有效的防治措施。本研究针对前期分离到的根结线虫高效生防菌粉红螺旋聚孢霉ClonostachysroseaNF-06菌株,以微胶囊的包埋率为指标,通过单因素试验和正交试验优化了其微胶囊制剂的复合壁材配方,进一步评价了微胶囊的货架期稳定性,逆境耐受性以及对番茄根结线虫病的室内防治效果。结果显示,在粉红螺旋聚孢霉发酵液接种量为30%,助剂海藻寡糖浓度为0.5%,CaCl_(2)浓度为3%,交联固化时间为24 h的条件下,当海藻酸钠添加量为2%,淀粉添加量3%,膨润土添加量为3%时,微胶囊制剂的活菌数最多6.3×10^(8)CFU/g,包埋率最高95.5%。此外,与发酵液相比,微胶囊制剂显著提高了粉红螺旋聚孢霉的货架期、耐盐性、耐酸碱性、耐高温和耐紫外照射能力,并且显著增强了菌株对番茄根结线虫病的防治效果和对番茄的促生效果。本研究为生物杀线剂的开发和利用提供了科学依据。With the rapid development of protected cultivation in the vegetable industry,the occurrence of root-knot nematode has become increasingly severe.Biological control is an effective management strategy for managing this issue.This study focuseed on Clonostachys rosea NF-06,which was screened as biocontrol agent of root-knot nematode previously.Using encapsulation rate as an indicator,the study optimized the proportion of wall materials through single-factor and orthogonal experiments.Further evaluation was conducted focused on the shelf-life stability,stress tolerance,and their control efficiency against Meloidogyne incognita in pot.The results showed that under the conditions of 30%inoculum dosage of C.rosea fermentation broth,0.5%seaweed oligosaccharide,3%CaCl_(2),and a cross-linking curing time of 24 hours,the addition of 2%sodium alginate,3%starch,and 3%bentonite yielded the highest number of spore contents and encapsulation rate in the microcapsule formulation,reaching 6.3×10^(8)CFU/g and 95.5%,respectively.Additionally,the preparation of microcapsule significantly enhanced the shelf life,salt tolerance,acid and alkaline resistance,heat resistance,and UV irradiation resistance of C.rosea when compared to the fermentation broth.Furthermore,it improved the strain's efficacy in controlling tomato root-knot nematode and growth promoting on tomatoes.This study provides a scientific basis for the development and application of bio-nematicides.

关 键 词：粉红螺旋聚孢霉 微胶囊 南方根结线虫 货架期 抗逆性 

分 类 号：S476[农业科学—农业昆虫与害虫防治]