生防菌ZF516与化学药剂复配种衣剂对豇豆根腐病防治的增效作用  

Synergistic Effect of Compound Seed Coating Containing Biocontrol Bacteria ZF516 and Chemical Agent on the Control of Cowpea Fusarium Root Rot

作　　者：赵子璇 石彬 郎均英 姜涛 杨静静 范腾飞 谢学文[1] 石延霞[1] 柴阿丽[1] 李宝聚[1,3] 李磊 ZHAO Zixuan;SHI Bin;LANG Junying;JIANG Tao;YANG Jingjing;FAN Tengfei;XIE Xuewen;SHI Yanxia;CHAI Ali;LI Baoju;LI Lei(State Key Laboratory of Vegetable Biobreeding/Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;The Agricultural Technology Extension Center of Fuyang District in Hangzhou,Hangzhou 311403,China;China Vegetable Biotechnology(Shouguang)Co.,Ltd,Weifang 262700,China)

机构地区：[1]中国农业科学院蔬菜花卉研究所,蔬菜生物育种全国重点实验室,北京100081 [2]杭州市富阳区农业技术推广中心,杭州311403 [3]中蔬生物科技(寿光)有限公司,潍坊262700

出　　处：《中国生物防治学报》2025年第1期165-176,共12页Chinese Journal of Biological Control

基　　金：国家重点研发计划(2023YFD1401200);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS);农业农村部园艺作物生物学与种质创制重点实验室(IVF2023);蔬菜生物育种全国重点实验室开放课题。

摘　　要：为了筛选出对豇豆根腐病具有显著拮抗效果的生防细菌,本研究采用平板对峙法获得了一株对豇豆根腐病复合致病菌均表现出显著抑制作用的生防菌株ZF516。随后,对该菌株进行了形态学特征分析,并对其16SrRNA、gyr A及rpo A基因序列进行了比对分析,明确菌株ZF516的分类地位。通过相容性和菌药复配比例筛选实验,将菌株ZF516分别与23%吡虫·咯·苯甲和29%噻虫·咯·霜灵进行复配,评估其对豇豆的生物安全性和根腐病防治效果。结果表明,菌株ZF516对茄病镰孢菌Fusarium solani、尖孢镰刀菌F.oxysporum和层出镰孢菌F.proliferatum的抑制率分别为71.83%、66.31%和73.73%,并被鉴定为贝莱斯芽胞杆菌Bacillusvelezensis;贝莱斯芽胞杆菌ZF516与23%吡虫·咯·苯甲以7:3体积比复配后,包衣处理的豇豆出苗率高于裸种和各自单独包衣处理,对豇豆根腐病的防治效果可达67.56%,优于化学种衣剂单独包衣处理;同时,贝莱斯芽胞杆菌ZF516与23%吡虫·咯·苯甲悬浮种衣剂复配后,对豇豆根腐病的防治表现出协同增效作用。本研究筛选获得了一株贝莱斯芽胞杆菌ZF516,其在单独使用和复配药剂后对豇豆根腐病均具有良好的抗病效果,为豇豆根腐病生物种衣剂的研发提供了重要的菌株资源和综合防治策略。In order to obtain biocontrol bacteria with remarkable antagonistic effects against cowpea root rot,in this study,the dural culture method was utilized to isolate a biocontrol strain,ZF516,which exhibited significant inhibitory effects on the complex pathogenic bacteria of cowpea root rot.Subsequently,the morphological characteristics of this strain were analyzed,and the 16S rRNA,gyrA,and rpoA gene sequences were compared and analyzed to clarify the taxonomic status of strain ZF516.The biosafety and root rot control efficacy of cowpea were evaluated through compatibility and compounding ratio screening of ZF516 with 23%imidacloprid·fludioxonil·difenoconazole and 29%thiamethoxam·fludioxonil·metalaxyl-M,respectively.The results indicated that the inhibition rates of strain ZF516 against Fusarium solani,F.oxysporum,and F.proliferatum were 71.83%,66.31%,and 73.73%.The strain ZF516 was identificated as Bacillus velezensis.After formulating Bacillus velezensis ZF516 and 23%imidacloprid-thiamethoxam-benomyl at a volume ratio of 7:3,the emergence rate of cowpea seeds treated with this combined seed coating was significantly higher compared to untreated seeds and seeds treated with each agent individually.Additionally,the compound of Bacillus velezensis ZF516 with 23%imidacloprid·thiamethoxam·benomyl suspension seed coating agent showed a synergistic effect on the control of cowpea root rot,achieving a control efficiency of up to 67.56%,which surpassed the efficacy observed with the chemical seed coating agent alone.In this study,a strain of Bacillus velezensis ZF516 was isolated and characterized.ZF516 exhibited significant disease resistance against cowpea root rot,both as a standalone treatment and in combination with chemical agents.This study provides essential strain resources and integrated control strategies for the development of biological seed coating agents aimed at managing cowpea root rot.

关 键 词：种衣剂 豇豆根腐病 芽胞杆菌 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]