牦牛颗粒细胞体外培养方案的优化  

Optimization of in Vitro Culture Method for Yak Granulosa Cells

作　　者：舒适 SHU Shi(Academy of Animal and Veterinary Science,Qinghai University,Xining 810016,China)

机构地区：[1]青海大学畜牧兽医科学院,青海西宁810016

出　　处：《青海畜牧兽医杂志》2025年第1期21-25,共5页Chinese Qinghai Journal of Animal and Veterinary Sciences

摘　　要：牦牛繁殖率低是制约牦牛经济效益的主要原因之一。为了深入研究牦牛繁殖的分子机制,本研究使用3种不同方案分别对牦牛颗粒细胞进行培养,最终优化筛选出生长速度快、传代效果好的牦牛颗粒细胞培养方案。相对于其他两种培养方案,用含有10%双抗的生理盐水浸泡的方法将采集的卵巢带回实验室,并使用含有10%胎牛血清的完全培养基对颗粒细胞进行培养后的培养效果最佳。本研究为提高牦牛繁殖率的分子策略研究提供了理论依据和数据支撑。The low reproductive rate of yaks is one of the main issues that restrict their economic benefits.In order to further study the molecular mechanisms of yak reproduction,this study optimized the culture scheme of yak granulosa cells with fast growth rate and good passage effect by using different schemes.Compared with the other two cultivation methods,the method of soaking the collected ovaries in physiological saline containing 10%bispecific antibodies and culturing them in complete medium containing 10%fetal bovine serum showed the best effect on the later cultivation of granulosa cells.This study provides theoretical basis and data support for molecular research to improve yak reproductive rate.

关 键 词：牦牛 颗粒细胞 体外培养 优化 

分 类 号：S823.85[农业科学—畜牧学]