六种卷柏属药材的抗炎活性及有效成分分析  

Analysis of Anti-inflammatory Activities and Effective Components of Six Species of Selaginella

作　　者：吴优 张学艳 李子寒 许苏 陈科力 李娟 WU You;ZHANG Xueyan;LI Zihan;XU Su;CHEN Keli;LI Juan(School of Pharmacy,Hubei University of Chinese Medicine,Wuhan 430065,China)

机构地区：[1]湖北中医药大学药学院,武汉430065

出　　处：《世界科学技术-中医药现代化》2025年第1期143-153,共11页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：国家自然科学基金委员会青年基金(81903921):江南卷柏黄酮调控NLRP3炎症小体抑制细胞焦亡治疗痛风的作用机制,负责人:李娟。

摘　　要：目的比较六种卷柏属药材的抗炎活性,并分析其有效部位和成分。方法用紫外和高效液相色谱分别测定卷柏属药材各提取部位总黄酮和乙酸乙酯部位主要成分的含量。采用刀豆蛋白和脂多糖诱导大鼠脾脏淋巴细胞和小鼠巨噬细胞构建体外炎症细胞模型,用CCK-8法测定细胞活性,Griess法测定一氧化氮(Nitric oxide,NO)水平,ELISA法测定IL-1β、TNF-α含量,RT-PCR检测诱导型一氧化氮合酶(Inducible nitric oxide synthase,iNOS)和TNF-α等炎症因子mRNA表达情况。结果卷柏属药材乙酸乙酯部位的黄酮含量相对较高,穗花杉双黄酮等双黄酮为其主要成分。六种卷柏属药材的各提取部位均无明显的细胞毒作用,乙酸乙酯部位、罗伯斯塔双黄酮-4'-甲醚和穗花杉双黄酮等均能显著降低RAW264.7细胞或淋巴细胞NO释放量,两种模型均以细叶卷柏乙酸乙酯部位的药效更为显著。细叶卷柏的双黄酮类成分类型较为丰富,罗伯斯塔双黄酮类成分含量相对高于其它品种,作用机制与其抑制IL-1β、TNF-α等炎症因子水平、下调TNF-α、IL-6、IL-1β以及iNOS等的mRNA表达有关。结论六种卷柏属药材均具有一定的体外抗炎作用,以细叶卷柏的活性相对更强,双黄酮类成分为其主要有效成分。Objective The anti-inflammatory activity of medicinal plants in the six species of Selaginella genus were compared,and their effective parts and components were analyzed.Methods The total flavonoid content of various extraction parts and the main components of ethyl acetate part of Selaginella were determined by ultraviolet and highperformance liquid chromatography(HPLC),respectively.In vitro inflammatory cell models were constructed using Concanavalin A(ConA)and lipopolysaccharide(LPS)induced rat splenic lymphocytes and mouse macrophages,and cell viability was measured by CCK-8 methods.The nitric oxide(NO)level was determined by the Griess method,and the levels of IL-1βand TNF-αwere determined by ELISA.The mRNA expression of inducible nitric oxide synthase(iNOS)and TNF-α,among other inflammatory factors,were detected by RT-PCR.Results The flavonoid content of the ethyl acetate part of Selaginella was relatively high,and biflavonoids such as amentoflavone was the main components.All the extraction parts of the six species of Selaginella showed no significant cytotoxicity.The ethyl acetate part,roberstonin-4'-methylether,and amentoflavone could significantly reduce the release of NO in RAW264.7 cells or lymphocytes,and the ethyl acetate part of Selaginella labordei were the most effective in both models.Selaginella labordei had a rich variety of biflavonoid components,with roberstonin-type biflavonoids relatively higher than other species.The mechanism of action was related to the inhibition of inflammatory factors such as IL-1βand TNF-αlevels,the downregulation of mRNA expression of TNF-α,IL-6,IL-1β,iNOS,and others.Conclusion All six species of Selaginella have certain in vitro anti-inflammatory effects and Selaginella labordei showed stronger activity,with biflavonoids as their main active components.

关 键 词：卷柏属 抗炎 RAW264.7细胞 淋巴细胞 双黄酮 

分 类 号：R284[医药卫生—中药学]