惠州地区扶桑绵粉蚧寄主危害情况及入侵来源  

Invasion source and phylogenetic analysis of the invasive mealybug Phenacoccus solenopsis in Huizhou

作　　者：吴福中 吴晟晗 吴玥桐 刘志红 WU Fuzhong;WU Shenghan;WU Yuetong;LIU Zhihong(Shenzhen City Polytechnic(Shenzhen Institute of Technology),Shenzhen,Guangdong 518116,China;Affiliated School of Huizhou University,Huizhou,Guangdong 516007,China;Agricultural Technology Promotion Center at Huicheng District Huizhou City,Huizhou,Guangdong 516001,China)

机构地区：[1]深圳城市职业学院(深圳技师学院),广东深圳518116 [2]惠州学院附属学校,广东惠州516007 [3]惠州市惠城区农业技术推广中心,广东惠州516001

出　　处：《生物安全学报(中英文)》2025年第1期34-39,共6页Journal of biosafety

基　　金：广东省科技项目(2019A141401001);广东省科技奖入库培育项目(2021C0405004);广东省普通高校工程技术研究中心项目(2024GCZX016);惠州市科技项目(2017C0424040);深圳技师学院博士启动金项目(2314001)。

摘　　要：【目的】弄清扶桑绵粉蚧在惠州地区的发生危害和入侵来源。【方法】对惠州地区扶桑绵粉蚧发生危害进行系统调查,并对采集的样品开展形态学鉴定、DNA提取和PCR扩增,利用生物信息学软件分析其序列同源性、遗传结构及系统进化关系。【结果】惠州地区扶桑绵粉蚧危害的寄主植物有15科43种,危害具有重要经济价值的马铃薯和蔬菜等农作物。测出的线粒体COⅠ基因序列有4个单倍型,在GenBank数据库中比对,序列相似度达到100%,种群间遗传距离为0~0.029,其中,惠州种群与美国种群遗传距离较大,与其他种群遗传距离较小。构建的系统发育树显示,扶桑绵粉蚧惠州种群与巴基斯坦种群聚在一支。【结论】推测惠州地区发生的扶桑绵粉蚧可能来源于巴基斯坦,该结论为惠州马铃薯和蔬菜产区监测该虫的发生和精准防治提供科学依据。【Aim】To investigate the occurrence and invasion source of Phenacoccus solenopsis in Huizhou,an important invasive pest that invaded China in recent years.【Method】In this study,we investigated the infestation of P.solenopsis in Huizhou on collected samples using morphological identification,DNA extraction,and PCR amplification to analyze its sequence homology,genetic structure,and phylogenetic relationship with bioinformatics software.【Result】There are 43 species of host plants in 15 families in Huizhou,which include potatoes,vegetables,and other economically important crops.Four haplotypes exist in the mitochondrial COⅠgene sequence,which were compared in the GenBank database.The sequence similarity reached 100%,and the genetic distance between populations was 0-0.029.The genetic distance between the Huizhou and American populations was large,whereas the genetic distance between the Huizhou and other populations was small.A phylogenetic tree showed that the Huizhou and Pakistani populations clustered together in one group.Based on the genetic distance and phylogenetic tree data,it was speculated that P.solenopsis in the Huizhou area may have originated from Pakistan.【Conclusion】The mitochondrial COⅠgene could quickly and accurately identify the invasion source and phylogenetic relationship of this insect,providing a scientific basis for monitoring the occurrence and precise control of this insect in potato and vegetable areas in Huizhou.

关 键 词：扶桑绵粉蚧 危害 入侵来源 系统发育分析 

分 类 号：S433.3[农业科学—农业昆虫与害虫防治]