基于SSR标记的牡丹品种鉴定技术标准体系的构建  

Development of identification standard system for tree peony varieties based on SSR markers

作　　者：张雨婷 于雪丹[2] 郑勇奇 张川红[2] 黄金锋[3] 赵国栋 李伟 李丰钰 ZHANG Yuting;YU Xuedan;ZHENG Yongqi;ZHANG Chuanhong;HUANG Jinfeng;ZHAO Guodong;LI Wei;LI Fengyu(College of Landscape Architecture and Forestry,Qingdao Agricultural University,Qingdao,Shandong 266109,China;Laboratory of Molecular Testing for New Plant Varieties,National Forestry and Grassland Administration/Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China;Heze Test Station for Forest and Grassland Plants,Heze Development Service Center of Peony,Heze,Shandong 274000,China;Luoyang National Peony Gene Bank,Luoyang Developmen Center for Peony Industry,Luoyang,Henan 471011,China)

机构地区：[1]青岛农业大学园林与林学院,山东青岛266109 [2]国家林业和草原局植物新品种分子测定实验室/中国林业科学研究院林业研究所,北京100091 [3]菏泽市牡丹发展服务中心国家林草植物新品种菏泽测试站,山东菏泽274000 [4]洛阳市牡丹产业发展中心洛阳国家牡丹基因库,河南洛阳471011

出　　处：《福建农林大学学报(自然科学版)》2025年第2期174-187,共14页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家林草局科技发展中心项目(KJZXZF2018004)。

摘　　要：【目的】建立牡丹品种鉴定的简单重复序列(SSR)标记标准技术体系,筛选出多态性高、重复性好的牡丹SSR核心引物和参照品种,并确定品种差异判定标准,为牡丹品种的精准快速鉴定及新品种权审查提供技术支持。【方法】以9个野生牡丹种和5个具有代表性品种群、9种花型及11种花色的48个常见栽培品种为试验材料,通过收集引物信息、核对DNA序列信息、重新设计引物、分析位点多态性,筛选出SSR核心引物和参照品种,并对SSR位点组合的品种鉴别力进行分析与评价。【结果】在10个SSR位点中,9个野生种和48个品种共检测到84个等位基因。等位基因数为4~12个;多态性信息含量为0.572~0.837,均值为0.712。SSR位点组合的品种鉴别能力分析结果显示:当差异位点为1个时,至少需要5对引物才可完全区分48个品种;而当差异位点为2个时,至少需要8对引物才能完全区分48个品种。【结论】筛选出的17个参照品种和10个SSR位点在所有牡丹品种群中均表现出良好的鉴别能力。最终确定了以1个差异位点为鉴定标准的牡丹品种SSR标记技术体系。【Objective】For rapid and precise identification of peony varieties and assessment of new variety rights,identification sys⁃tem and benchmark were established based on SSR marker through screening reference varieties and SSR core primers with high pol⁃ymorphism and good repeatability.【Method】With 9 wild species as well as 48 common cultivated varieties,belonging to 5 groups with diverse morphology and covering 9 flower types and 11 flower colors,as materials,core SSR primers and reference varieties were screened through primer information collection,DNA sequence data verification,primer redesign,and locus polymorphism a⁃nalysis.And the variety discrimination power(VDP)of SSR loci combinations were further examined and evaluated.【Result】A total of 84 alleles were identified from the 10 analyzed SSR loci of the 9 wild species and 48 cultivated varieties. The allele number(Na) per locus ranged from 4 to 12. The polymorphism information content (PIC) ranged from 0.572 to 0.837, with an average of0.712. The VDP assessment of SSR loci combinations indicated that a minimum of 5 primer pairs were necessary to fully distinguishall the 48 varieties when the number of variety⁃specific differential locus was 1, and at least 8 primer pairs were prerequisite whenthe number was 2. 【Conclusion】The screened 17 reference varieties and 10 pairs of SSR core primers showed sound variety discrimi⁃nation power in all the peony cultivar groups tested, and a SSR marker⁃based system with 1 differential locus as the benchmark wasdeveloped for the identification of peony varieties.

关 键 词：牡丹 SSR标记 品种鉴定 分子身份证 品种鉴别力 

分 类 号：S685.11[农业科学—观赏园艺]