低能耗原位铜电离技术对不同生长期水华蓝藻的杀灭效果及藻毒素风险分析  

Effect of low-energy in-situ copper ionization technology on the elimination of cyanobacterial blooms at different growth stages and the risk analysis of algal toxins

作　　者：刘晓亚 陈咨霖 谢楚蓉 曲同超 何凡星 陶雪 刘佩蕊 傅海燕 LIU Xiaoya;CHEN Zilin;XIE Churong;QU Tongchao;HE Fanxing;TAO Xue;LIU Peirui;FU Haiyan(School of Environmental Science and Engineering,Xiamen University of Technology,Xiamen 361024,China;College of Environment,Zhejiang University of Technology,Hangzhou 310014,China;Department of Resources and Environment,Moutai Institute 564507,China)

机构地区：[1]厦门理工学院环境科学与工程学院,厦门361024 [2]浙江工业大学环境学院,杭州310014 [3]茅台学院资源环境系,仁怀564507

出　　处：《环境工程学报》2024年第12期3528-3537,共10页Chinese Journal of Environmental Engineering

基　　金：福建省中青年教师教育科研项目资助(JAT231103);厦门理工学院高层次人才资助项目(YKJ23004R);国家自然科学基金青年资助项目(42207517);贵州省科技计划资助项目(黔科合基础-ZK[2021]一般282)。

摘　　要：相较于传统化学杀藻剂依赖外源铜离子的直接投加,低能耗电场辅助原位铜电离技术通过电场作用实现铜离子的原位释放,增强铜离子直接作用于藻细胞的灭活效果,降低二次污染风险并提升水华藻治理的安全性。鉴于蓝藻水华在形成和发展过程中,不同生长期的藻细胞在生理特性和生长状态上存在显著差异,可能影响除藻技术的实际应用效果,本研究利用低能耗电场辅助原位铜电离装置,系统评估了该技术对不同生长期铜绿微囊藻的灭活效果,并分析了不同运行条件下对微囊藻毒素-LR(MC-LR)合成与释放的影响。研究结果表明:1)在电压为0.5 V的条件下,低流速(2 mL·min^(−1))处理相比于高流速(10 mL·min^(−1))显著提高了对数期和稳定期藻细胞的死细胞比例,分别增加了162.01%和278.02%;2)电压为1.0 V、流速为2 mL·min^(−1)的条件下,迟缓期和对数期藻细胞的氧化应激反应显著加剧,达到最佳灭藻效果;3)铜电离处理后的杀藻效果在再培养过程中仍能维持,对数期藻细胞经高电压低流速处理后,OD680-96 h抑制率可达40%,F_(v)/F_(m)值在48 h内从0.54降至0.42;4)铜电离对迟缓期和稳定期藻细胞具有较好的灭活效果,但同时增加了藻毒素释放的风险,而对对数期藻细胞不仅实现了有效灭活,同时减少了胞内MC-LR的含量。该研究不仅为低能耗原位铜电离技术应用于蓝藻水华不同时期的精准灭活提供了有效策略,还为降低藻毒素风险、确保水体生态安全提供了重要的理论依据和实践指导。Compared with traditional chemical algaecides that rely on the direct addition of exogenous copper ions,the low-energy in situ copper ionization technology utilizes an electric field to induce the release of copper ions in situ,increasing the direct killing effect on algae cells,and reducing the risk of secondary pollution and improving the safety of cyanobacterial bloom control.Considering the significant differences in physiological characteristics and growth states of cyanobacterial cells at different growth stages during the formation and development of blooms,which may affect the algae killing efficacy,this study systematically evaluated the inactivation efficiency of this technology on Microcystis aeruginosa at various growth stages.In addition,the synthesis and release of microcystin-LR(MC-LR)under different operating conditions were analyzed.The results showed that:1)At a voltage of 0.5 V,treatment with a low flow rate(2 mL·min^(−1))significantly increased the proportion of dead cells in the logarithmic and stationary phases by 162.01%and 278.02%,respectively,compared to a high flow rate(10 mL·min^(−1));2)At a voltage of 1.0 V and a flow rate of 2 mL·min^(−1),oxidative stress in cells during the lag and logarithmic phases was significantly enhanced,achieving the optimal algal inactivation effect;3)The algal inactivation effect of copper ionization was sustained during recultivation,with a OD680-96 h inhibition rate of 40%for logarithmic phase cells treated with high voltage and low flow rate,and the F_(v)/F_(m)value decreased from 0.54 to 0.42 within 48 h;4)Copper ionization exhibited good inactivation effects on cells in the lag and stationary phases,but it also increased the risk of microcystin release.In contrast,it effectively inactivated logarithmic phase cells while reducing intracellular MC-LR content.This study not only provides effective strategies for the precise inactivation of cyanobacterial blooms at different growth stages using low-energy in-situ copper ionization technology,but al

关 键 词：蓝藻水华 铜绿微囊藻 铜电离 光合活性 氧化应激 微囊藻毒素 

分 类 号：X524[环境科学与工程—环境工程]