补肾强督方改善DBA/1小鼠新骨形成的机制  

Mechanism of Bushen Qiangdu Formula in osteogenesis of DBA/1 mice

作　　者：李艳萍 夏启胜[2] 朱峰 阎小萍[3,4] 陶庆文[3,4] 孔维萍[3,4] LI Yanping;XIA Qisheng;ZHU Feng;YAN Xiaoping;TAO Qingwen;KONG Weiping(Beijing University of Chinese Medicine,Beijing 100029,China;Institute of Clinical Medicine,China-Japan Friendship Hospital,Beijing 100029,China;Department of TCM Rheumatology,China-Japan Friendship Hospital,Beijing 100029,China;Beijing Key Lab for Immune-Mediated Inflammatory Diseases,China-Japan Friendship Hospital,Beijing 100029,China)

机构地区：[1]北京中医药大学,北京100029 [2]中日友好医院临床医学研究所,北京100029 [3]中日友好医院中医风湿病科,北京100029 [4]中日友好医院,北京免疫介导炎症性疾病重点实验室,北京100029

出　　处：《中华中医药杂志》2025年第1期365-370,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金项目(No.81403378);北京市自然科学基金项目(No.7182148);中央高水平医院临床科研业务费资助(No.2022-NHLHCRF-LX-02-0104)。

摘　　要：目的:研究补肾强督方(BSQD)对强直性脊柱炎(AS)成骨过程中Wnt/β-catenin信号通路的影响。方法:40只DBA/1小鼠分为模型(AS)组、西药对照(CELEBREX)组、补肾强督方低剂量(LBSQD)组及补肾强督方高剂量(HBSQD)组,每组10只,另取10只C57BL/6小鼠作为空白对照(Normal)组。评估小鼠的关节炎评分。对小鼠关节进行HE染色以评估骨化。实时荧光定量PCR和Western Blot、免疫组织化学分别分析SOST、Wnt3a、β-catenin mRNA和蛋白的表达。结果:与Normal组比较,DBA/1小鼠表现出AS样关节炎。BSQD、CELEBREX组显著降低AS关节炎评分。组织病理学显示,AS组评分显著高于正常对照组(P<0.05),而HBSQD组评分显著低于AS组(P<0.05)。AS组和HBSQD组的SOST mRNA和蛋白表达分别高于Normal组和AS组(P<0.05,P<0.01)。与Normal组比较,AS组Wnt3a mRNA和蛋白表达增加(P<0.05),而HBSQD组Wnt3a mRNA和蛋白质表达低于AS组(P<0.05,P<0.01)。β-catenin在HBSQD、LBSQD和CELEBREX组中的表达显著低于AS组(P<0.05,P<0.01)。结论:补肾强督方能抑制AS的Wnt/β-catenin信号通路,抑制成骨。Objective:To study the effects of Bushen Qiangdu Formula(BSQD) on the Wnt/β-catenin signaling pathway during osteogenesis of ankylosing spondylitis(AS).Methods:A total of 40 DBA/1 mice were randomly divided into AS,CELEBREX,LBSQD and HBSQD group,10 mice in each group,with 10 C57BL/6 mice as the Normal group.Evaluate the arthritis score of mice.Perform hematoxylin-eosin staining on mouse joints to assess the ossification scores.The mRNA and protein expression of SOST,Wnt3a,and β-catenin were analyzed by RT-PCR,immunohistochemistry,and Western Blot.Results:Compared with the Normal group,DBA/1 mice showed AS-like arthritis.BSQD and CELEBREX significantly reduce the AS arthritis score.The Achilles tendon histopathology showed that the AS group score was significantly higher than that of the Normal group(P<0.05),while the HBSQD group score was significantly lower than that of the AS group(P<0.05).The expression of SOST mRNA and protein was higher in the AS group and the BSDQ group than in the Normal group and AS group,respectively(P<0.05,P<0.01).Compared to the Normal group,Wnt3a mRNA and protein expression increased in the AS group(P<0.05),while it was ower in the HBSQD group compared to the AS group(P<0.05,P<0.01).The expression of β-catenin in the HBSQD,LBSQD and CELEBREX groups was significantly lower than in the AS group(P<0.05,P<0.01).Conclusion:BSQD can inhibit the Wnt/β-catenin signaling pathway of AS and inhibit osteogenesis.

关 键 词：强直性脊柱炎 DBA/1 WNT/Β-CATENIN通路 新骨形成 补肾强督方 

分 类 号：R285.5[医药卫生—中药学]