一测多评法同时测定瑶药董殃咪中5个成分的含量  

Simultaneous determination of five components in Yao medicine Galium aparine by quantitative analysis of multi-components with single marker

作　　者：李耀华[1] 谢洁兰 雷沛霖 谢鲜丽 卿丽婷 梁建丽[1] 潘真真 李芳婵 陆峥琳 黄瑞松 LI Yaohua;XIE Jielan;LEI Peilin;XIE Xianli;QING Liting;LIANG Jianli;PAN Zhenzhen;LI Fangchan;LU Zhenglin;HUANG Ruisong(Guangxi University of Chinese Medicine,Key Laboratory of TCM Extraction and Purification and Quality Analysis,National Demonstration Center for Experimental Chinese Medicine Education,Nanning 530200,China;Guangxi International Zhuang Medicine Hospital,HUANG Ruisong’s Inheritance Studio of National Veteran Experts of TCM,Nanning 530201,China)

机构地区：[1]广西中医药大学,广西高校中药提取纯化与质量分析重点实验室,中药学国家级实验教学示范中心,南宁530200 [2]广西国际壮医医院,黄瑞松全国名老中医专家传承工作室,南宁530201

出　　处：《中华中医药杂志》2025年第1期385-389,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：广西科技计划项目重点研发计划(No.桂科AB18221099);广西中医药大学广西一流学科建设开放课题(No.2019XK107);广西壮族自治区瑶药材质量标准(第二卷)质量评价与标准研究项目(No.MZY2017004);广西中医药大学赛恩斯新医药学院大学生创新创业训练计划项目(No.201913643053);国家中医药管理局全国名老中医药专家传承工作室建设项目——黄瑞松全国名老中医药专家传承工作室(No.国中医药人教函[2022]75号);广西中药壮瑶药炮制技术重点研究室(No.桂中医药科教发[2023]9号)。

摘　　要：目的:建立瑶药董殃咪中5个成分(绿原酸、新绿原酸、隐绿原酸、异绿原酸A及异绿原酸C)的一测多评含量测定方法。方法:采用广州菲尼根X-Peonyx C_(18)色谱柱(4.6 mm×250 mm,5μm),甲醇-0.1%甲酸为流动相,梯度洗脱;检测波长327 nm,柱温30℃。以绿原酸为内标物,采用多点校正法,分别建立与其余成分相对校正因子,并计算8批瑶药董殃咪样品的4个待测物的含量,与外标法进行比较,验证一测多评法的准确性和可行性。结果:新绿原酸、隐绿原酸、异绿原酸A及异绿原酸C相对校正因子分别为1.045、0.927、1.292、1.198,一测多评法与外标法无显著差异。结论:建立的瑶药董殃咪的一测多评法,准确可行,简便稳定,可节省大量对照品使用,为该药材的质量控制提供理论基础。Objective:To establish a method for the evaluation of 5 components(chlorogenic acid,neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A and isochlorogenic acid C) in Yao medicine Galium aparine through quantitative analysis of multi-components with single marker(QAMS).Methods:Feinigen X-Peonyx C18 column(4.6 mm×250 mm,5 μm)was used,the mobile phase was methanol-0.1% formic acid aqueous solution for gradient elution,the detection wavelength was 327 nm,and the column temperature was 30 ℃.With chlorogenic acid as the internal standard,the relative correction factors with other components were established by multi-point correction method,and the contents of 4 substances to be measured in 8 batches of Yao medicine Galium aparine samples were calculated,and compared with the external standard method,to verify the accuracy and feasibility of the QAMS method.Results:The relative correction factors of neochlorogenic acid,cryptochlorogenic acid,isochlorogenic acid A and isochlorogenic acid C were 1.045,0.927,1.292,1.198,respectively.The results of QAMS method had not significantly differents compared with the external standard method.Conclusion:The QAMS method is accurate and feasible,simple and stable,and can save a lot of reference substances and provide a theoretical basis for the quality control of Yao medicine Galium aparine.

关 键 词：董殃咪 相对校正因子 一测多评 绿原酸 新绿原酸 隐绿原酸 异绿原酸A 异绿原酸C 

分 类 号：O657.72[理学—分析化学] R29[理学—化学]