基于16S rDNA测序研究揿针治疗对变应性鼻炎患儿舌苔菌群多样性的影响  

Study on the effects of pressing needle therapy on the diversity of tongue coating microbiota in children with allergic rhinitis based on 16S rDNA sequencing

作　　者：余阳[1] 刘庆 陈秋彤 李铁林 张雨桐 倪夕秀 黄思莹 赵凌[1] YU Yang;LIU Qing;CHEN Qiutong;LI Tielin;ZHANG Yutong;NI Xixiu;HUANG Siying;ZHAO Ling(School of Acupuncture and Tuina,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075

出　　处：《中华中医药杂志》2025年第1期451-456,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家中医药管理局青年岐黄学者支持项目;四川省科技厅中央引导地方科技发展资金项目(No.2021ZYD0103);成都中医药大学“杏林学者”学科人才科研提升计划项目(No.JQRC20210001)。

摘　　要：目的:观察揿针治疗变应性鼻炎(AR)患儿的疗效和舌苔菌群变化特征。方法:纳入AR患儿28例为鼻炎组,予以揿针治疗4周,每周2次。健康儿童24例为健康组,不予以任何治疗。采集受试者舌苔进行16S rDNA测序分析,观察治疗前后的鼻部症状评分(TNSS)与儿童鼻结膜炎相关生活质量问卷评分(PRQLQ),并对比鼻炎组治疗前与治疗后及健康组的舌苔菌群差异。结果:鼻炎组Chao1值较健康组显著降低(P<0.05),空肠普雷沃氏菌种、拉瓦拟普雷沃氏菌种、淡色普雷沃氏菌种显著减少(P<0.05)。治疗后,鼻炎组的TNSS和PRQLQ评分显著降低(P<0.05),空肠普雷沃氏菌种、拉瓦拟普雷沃氏菌种、淡色普雷沃氏菌种显著升高(P<0.05)。其中空肠普雷沃氏菌种与TNSS、PRQLQ评分负相关性最高。结论:AR患儿的舌苔菌群丰度明显低于健康儿童。揿针治疗后能显著改善患儿症状和生活质量,并且增加有益菌的丰度,在一定程度上调节舌苔菌群的结构来改善AR的临床症状。Objective:To observe the therapeutic effect of pressing needle therapy on children with allergic rhinitis(AR) and the characteristics of changes in tongue coating microbiota.Methods:Twenty-eight children with AR were included as the rhinitis group and were treated with pressing needle twice a week for 4 weeks.While 24 healthy children were included as the healthy group and didn't receive any treatment.The tongue coating of all subjects was collected for 16S rDNA sequencing analysis.The total nasal symptoms score(TNSS) and the pediatrics rhinoconjunctivitis quality of life questionnaire(PRQLQ) in the rhinitis group were compared before and after treatment.The changes in tongue coating microbiota between the rhinitis group and the healthy control group was observed before and after treatment.Results:The Chao1 value of tongue coating microbiota in the rhinitis group was significantly reduced compared to the healthy group(P<0.05),while the abundance of Prevotella_jejuni,Alloprevotella_rava,Prevotella_pallens were significantly decreased(P<0.05).After treatment,the TNSS and PRQLQ socres in the rhinitis group were significantly reduced(P<0.05),and there was a significant increase in the abundance of Prevotella_jejuni,Alloprevotella_rava,Prevotella_pallens after treatment(P<0.05).The Prevotella_jejuni showed the most significant negative correlation with the TNSS and PRQLQ scores.Conclusion:The abundance of tongue coating microbiota in AR children is significantly lower than that in healthy children.In addition,pressing needle therapy can significantly improve the symptoms and quality of life of AR children,and increase the abundance of beneficial bacteria,which can to some extent regulate the structure of the tongue coating microbiota to improve the clinical symptoms of AR.

关 键 词：变应性鼻炎 儿童 揿针 舌苔菌群 差异菌群 

分 类 号：R246.81[医药卫生—针灸推拿学]