基于环境DNA宏条形码技术的黄河三角洲典型潮沟单元秋季无脊椎动物多样性及共现网络分析  

Analysis of invertebrate diversity and co-occurrence network based on environmental DNA metabarcoding in autumn typical tidal creek units of the Huanghe River Delta

作　　者：符清露 董志远 李宝泉[2,3] 陈莉 孙德斌 倪艳梅 唐永政 陈琳琳[2,3] Fu Qinglu;Dong Zhiyuan;Li Baoquan;Chen Li;Sun Debin;Ni Yanmei;Tang Yongzheng;Chen Linlin(School of Ocean,Yantai University,Yantai 264003,China;Key Laboratory of Coastal Biology and Bioresource Utilization,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Yantai 264003,China;Key Laboratory of Coastal Zone Environmental Process and Ecological Restoration(Yantai Coastal Zone Research Institute),Chinese Academy of Sciences,Yantai 264003,China)

机构地区：[1]烟台大学海洋学院,山东烟台264003 [2]中国科学院烟台海岸带研究所海岸带生物学与生物资源保护实验室,山东烟台264003 [3]中国科学院海岸带环境过程与生态修复重点实验室(烟台海岸带研究所),山东烟台264003

出　　处：《海洋学报》2024年第11期75-90,共16页

基　　金：国家自然科学基金(42176160和42276142);山东省自然科学基金(ZR2021MD084);山东省水文中心黄河三角洲水文连通及其对湿地生态系统健康驱动研究(SDGP370000000202302007881A 001)。

摘　　要：潮沟系统作为滨海湿地中活跃的地貌单元,不同级别潮沟水文环境变化显著,进而导致生物群落的空间分布出现差异。本研究选取黄河三角洲典型潮沟单元,利用环境DNA宏条形码(environmental DNA metabarcoding,eDNA)技术检测不同级别潮沟无脊椎动物多样性,采用生物共现网络分析和冗余分析(RDA)分别揭示典型潮沟无脊椎动物关键种与驱动因素。结果表明:该潮沟单元共检测到无脊椎动物127个分类操作单元(Operational Taxonomic Units,OTUs),隶属于9门24纲53目103科87属90种。无脊椎动物门水平和属水平分别以节肢动物门(43.9%)和围沙蚕属(Perinereis)(25.2%)为优势类群。群落综合多样性指标(Comprehensive diversity index,CD)分析显示,三级潮沟综合多样性最高,一级潮沟无脊椎动物综合多样性最低。生物共现网络分析显示,线围沙蚕(Perinereis linea)和双枝薮枝螅(Obelia dichotoma)为关键种,对维持该潮沟无脊椎动物群落结构稳定起关键作用。RDA显示,水体的硅酸盐含量、温度和沉积物的粉砂、黏土占比是影响该潮沟无脊椎动物群落特征的主要环境因子。相关性网络分析显示,关键种受硅酸盐含量、黏土和水体氮元素含量的显著影响(P <0.05)。研究结果有助于了解黄河三角洲典型潮沟无脊椎动物群落结构,揭示典型潮沟无脊椎动物关键种,并为无脊椎动物多样性监测与保护提供数据支持与理论参考。The tidal creek system is an active geomorphic unit in coastal wetlands,and the water environment of different level tidal creeks changes significantly,leading to spatial distribution differences of biological communities.This study selected a typical tidal creek unit in the Huanghe River Delta and used environmental DNA metabarcoding(eDNA)technique to detect the diversity of invertebrates.The biological co-occurrence network analysis and redundancy analysis(RDA)were respectively used to reveal the keystone species and driving factors of the invertebrate community in the typical tidal creek.The results showed that a total of 127 operational taxonomic units(OTUs)of invertebrates were detected in the tidal creek unit,belonging to 9 phyla,24 classes,53 orders,103 families,87 genera,and 90 species;among them,the class level was dominated by the Arthropoda(43.9%),and the genus level was dominated by the Perinereis(25.2%).The comprehensive diversity index(CD)analysis showed that the comprehensive diversity of invertebrates in the third-level tidal creek was the highest,and the comprehensive diversity of invertebrates in the first-level tidal creek was the lowest.The biological co-occurrence network analysis showed that the Perinereis linea and the Obelia dichotoma were the keystone species,which played a key role in maintaining the stability of the invertebrate community structure in the tidal creek.The RDA showed that the silicate content of the water body,temperature,and the proportion of fine sand and clay in the sediment were the main environmental factors affecting the invertebrate community characteristics in the tidal creek.Correlation network analysis showed that the keystone species were significantly affected by silicate content,clay,and nitrogen content in water(P<0.05).The research results are helpful for understanding the community structure of typical tidal creek invertebrates,revealing the keystone species of typical tidal ditch invertebrates,and providing data support and theoretical reference for the m

关 键 词：环境DNA宏条形码 共现网络 生物多样性 无脊椎动物 黄河三角洲 

分 类 号：Q959.1[生物学—动物学]