FGF5和FGF21对绒山羊毛乳头细胞增殖的影响  

Effect of FGF5 and FGF21 on Proliferation of Dermal Papilla Cells in Cashmere Goat

作　　者：王妞 史昕冉 张卫东 王昕[1] WANG Niu;SHI XinRan;ZHANG WeiDong;WANG Xin(College of Animal Science and Technology,Northwest A&F University,Yangling 712100,Shaanxi)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国农业科学》2025年第4期819-830,共12页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31972556);陕西省自然科学基础研究计划(2019JZ-07)。

摘　　要：【背景】绒山羊毛囊周期分为生长期、退行期和休止期。成纤维生长因子5(fibroblast growth factor5, FGF5)和成纤维生长因子21(fibroblast growth factor21, FGF21)是毛囊周期转换的重要调控因子。然而,FGF5和FGF21在皮肤组织中的定位以及调控毛囊周期转换的作用机制仍不清楚,而且FGF21在毛囊发育过程中的作用尚存在争议。毛乳头细胞(dermal papilla cells, DPCs)位于毛囊的毛乳头区域,当其功能缺失时,毛囊不能发育,使毛囊周期停滞在休止期,导致脱发。【目的】通过检测FGF5和FGF21在绒山羊皮肤组织中的表达定位,探究FGF5和FGF21对DPCs增殖的影响,并对其调控机制进行解析,丰富成纤维生长因子家族基因调控毛囊周期转换的机制,为进一步阐明绒山羊毛囊周期转换的分子机制提供理论依据。【方法】利用实时荧光定量PCR(real time quantitative PCR, RT-qPCR)和酶联免疫吸附试验(enzyme-linked immunosorbent assay, ELISA)检测FGF5、FGF21及其受体1(fibroblast growth factor receptor1, FGFR1)在绒山羊生长期和休止期皮肤组织中的表达情况,结合单细胞转录组测序数据分析其在绒山羊皮肤组织中的表达定位,并利用组织免疫荧光技术进行验证;通过构建过表达腺病毒载体在绒山羊DPCs中过表达FGF5和FGF21,利用MTT、EdU、流式细胞周期等方法检测其对绒山羊DPCs增殖的影响,并利用RT-qPCR、细胞免疫荧光和Western blotting检测FGFR1及增殖相关基因的表达情况;进一步利用RT-qPCR、细胞免疫荧光和Western blotting等方法探究FGF5和FGF21影响绒山羊DPCs增殖的作用机制。【结果】FGF5、FGF21及其受体FGFR1在绒山羊生长期皮肤组织中的表达量显著高于休止期(P<0.05);FGF5、FGF21及其受体FGFR1均定位于绒山羊DPCs,提示FGF5和FGF21可能参与调控绒山羊DPCs的生物学功能;FGF5和FGF21过表达腺病毒在绒山羊DPCs中的过表达效果良好,其处理时间在84 h最佳(P<0.001);FGF5【Background】The hair follicle cycle in goat is divided into three phases:the growth phase(anagen),the regression phase(catagen),and the resting phase(telogen).Fibroblast growth factor 5(FGF5)and fibroblast growth factor 21(FGF21)are important regulators in goat hair cycle.However,the precise localization of FGF5 and FGF21 in goat skin tissue and their regulatory mechanisms remain unclear during hair follicle cycle transition.Dermal papilla cells(DPCs)located in the dermal papilla region,are essential for hair follicle development.When DPCs lose their function,hair follicle remains in the telogen,leading to hair loss.【Objective】Therefore,this study aimed to identify the expression and localization of FGF5 and FGF21 in Cashmere goat skin tissue,understand their roles in DPCs,and to analyze their regulatory mechanisms.This would enrich the knowledge of fibroblast growth factor family proteins in hair follicle cycle regulation and provide a theoretical basis for further elucidating the molecular mechanisms of Cashmere goat hair follicle cycle transition.【Method】Real-time quantitative PCR(RT-qPCR)and enzyme-linked immunosorbent assay(ELISA)were used to measure the expression levels of FGF5,FGF21 and their receptor 1(FGFR1)in the skin tissue of Cashmere goats at anagen and telogen.Single-cell transcriptome sequencing data were analyzed to determine the expression and localization of FGF5,FGF21 and FGFR1,which were validated through immunofluorescence methods.Adenovirus was explored to overexpress FGF5 and FGF21 in DPCs.The effects on DPCs proliferation were measured using MTT,EdU and flow cytometry.The expressions of FGFR1 and proliferation-related genes were detected using RT-qPCR,immunofluorescence and western blotting methods.RT-qPCR,immunofluorescence and western blotting were used to investigate the effect of FGF5 and FGF21 on the proliferation of DPCs.【Result】FGF5,FGF21 and FGFR1 exhibited higher expression levels in anagen than that in telogen,all of which were located within DPCs.Both FGF5 and F

关 键 词：绒山羊 FGF5 FGF21 DPCs 增殖 WNT/Β-CATENIN信号通路 

分 类 号：S827[农业科学—畜牧学]