清热祛浊胶囊对2型糖尿病合并非酒精性脂肪肝大鼠的治疗作用及机制  

Therapeutic effect and mechanism of Qingre Quzhuo Capsule on type 2 diabetes mellitus with non-alcoholic fatty liver in rats

作　　者：李华君[1] 吕树泉[1] 张辉 王元松[1] 苗静[2] 边育红 刘晓兰 LI Hua-Jun;LV Shu-Quan;ZHANG Hu(Hebei Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine,Cangzhou 061000,Hebei,China)

机构地区：[1]河北省沧州中西医结合医院,河北沧州061001 [2]天津市第二人民医院 [3]天津中医药大学中西医结合学院 [4]石家庄医学高等专科学校

出　　处：《中国老年学杂志》2025年第3期623-628,共6页Chinese Journal of Gerontology

基　　金：国家自然科学基金项目(82274424);王元松全国名老中医药专家传承工作室(冀中医药[2024]3号)。

摘　　要：目的研究清热祛浊胶囊对2型糖尿病(T2DM)合并非酒精性脂肪肝(NAFLD)模型大鼠的治疗及作用机制。方法取SD健康雄性大鼠50只,按随机数字表法平均分为正常组、模型组、阳性药组、清热祛浊胶囊低、高剂量组。正常组使用正常饮食饲养,其余各组运用高糖高脂饲料建立T2DM合并NAFLD模型。检测治疗后各组血糖、空腹胰岛素(FINS)、胰岛素抵抗指数(HOMA-IR)、血脂、肝肾功能水平,肝脏组织苏木素-伊红(HE)染色、油红O染色研究清热祛浊胶囊对T2DM合并NAFLD的治疗作用。运用试剂盒及酶联免疫吸附试验(ELISA)法研究清热祛浊胶囊对T2DM合并NAFLD大鼠肝组织中氧化应激及炎症反应的影响。应用实时荧光定量聚合酶链反应(RT-qPCR)和Western印迹检测各组肝组织中腺苷酸激活蛋白激酶(AMPK)、p-AMPK、固醇调节元件结合蛋白(SREBP)-1、乙酰辅酶A羧化酶(ACC)1、三磷酸腺苷柠檬酸裂解酶(ACLY)、脂肪酸合酶(FASN)mRNA及蛋白表达情况。结果清热祛浊胶囊干预后可显著降低T2DM合并NAFLD大鼠血糖、FINS、HOMA-IR、血脂、肝肾功能,并明显改善肝脏组织病理学变化(P<0.05,P<0.01)。清热祛浊胶囊可显著提高模型组血清中总超氧化物歧化酶(SOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,显著降低丙二醛(MDA)、白细胞介素(IL)-6、IL-1β及肿瘤坏死因子(TNF)-α水平(P<0.05,P<0.01)。与模型组相比,清热祛浊胶囊低、高剂量组可不同程度明显改善AMPK、p-AMPK、SREBP-1、ACC1、ACLY、FASN mRNA及蛋白表达(P<0.05,P<0.01)。结论清热祛浊胶囊对T2DM合并NAFLD具有治疗作用,其机制可能与AMPK/SREBP-1信号通路调控脂代谢有关。Objective To study the therapeutic effect and its mechanism of Qingre Quzhuo Capsule on type 2 diabetes mellitus(T2DM)with nonalcoholic fatty liver disease(NAFLD)in rats.Methods Fifty healthy male SD rats were randomly divided into normal group,model group,positive drug group,Qingre Quzhuo Capsule low and high dose groups.The normal group was fed with normal diet,and the other groups were fed with high-sugar and high-fat diet to establish T2DM combined with NAFLD model.The blood glucose,fasting insulin(FINS),insulin resistance index(HOMA-IR),blood lipids,liver and kidney function levels of each group after treatment were dected,hematoxylin-eosin(HE)staining,oil red O staining were used in liver tissue to study the therapeutic effect of Qingre Quzhuo Capsule on T2DM with NAFLD.The effect of Qingre Quzhuo Capsule on oxidative stress and inflammatory response in liver tissue of rats with T2DM and NAFLD was detected by kits and enzyme-linked immunosorbent assay(ELISA)method.Adenylateactivated protein kinase(AMPK),p-AMPK,and sterols regulatory element binding protein(SREBP)-1,acetyl-CoA carboxylase(ACC)1,adenosine triphosphate citrate lyase(ACLY),fatty acid synthase(FASN)mRNA and protein expressions in liver tissues of each group were detected by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)and Western blotting.Results After the intervention of Qingre Quzhuo Capsule,the blood glucose,FINS,HOMA-IR,blood lipid,liver and kidney function of T2DM rats with NAFLD were significantly decreased,and it improved the pathological changes of liver tissue(P<0.05,P<0.01).The total superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activity were significantly improved,the levels of malondialdehyde(MDA),interleukin(IL)-6,IL-1βand tumor necrosis factor(TNF)-αwere significantly decreased of serum in the model group by Qingre Quzhuo Capsule(P<0.05,P<0.01).Compared with the model group,AMPK,p-AMPK,SREBP-1,ACC1,ACLY and FASN mRNA and protein expressions were significantly improved in different degree by Q

关 键 词：清热祛浊胶囊 腺苷酸激活蛋白激酶(AMPK)/固醇调节元件结合蛋白(SREBP)-1 脂代谢 2型糖尿病 脂肪肝 

分 类 号：R587.2[医药卫生—内分泌]