黑龙江地区赤松梢斑螟越冬幼虫的耐寒性  

Cold tolerance of overwintering larvae of Dioryctria sylvestrella in Heilongjiang area

作　　者：王雪强 陈汝婷 丁锡垚 王美玲 邱云腾 周永亚 程金藏 杨志 宇佳[1] 迟德富[1] WANG Xueqiang;CHEN Ruting;DING Xiyao;WANG Meiling;QIU Yunteng;ZHOU Yongya;CHENG Jincang;YANG Zhi;YU Jia;CHI Defu(College of Forestry,Northeast Forestry University,Harbin 150040,Heilongjiang,China)

机构地区：[1]东北林业大学林学院,黑龙江哈尔滨150040

出　　处：《中南林业科技大学学报》2025年第1期70-81,共12页Journal of Central South University of Forestry & Technology

基　　金：“十四五”国家重点研发计划项目(2022YFD1401000);黑龙江省重点研发计划项目(2023ZX02B05)。

摘　　要：【目的】通过测定赤松梢斑螟Dioryctria sylvestrella越冬幼虫的抗寒能力和不同越冬阶段的生理生化指标,为赤松梢斑螟的越冬行为以及在我国的潜在地理分布等研究奠定理论基础。【方法】通过在越冬期剖树检查的方法确定赤松梢斑螟越冬幼虫龄期,使用过冷却点测定仪对赤松梢斑螟越冬幼虫的过冷却点和体液结冰点进行测定,并分析和幼虫龄期、体长、质量之间的关系,同时测定越冬期和越冬后期幼虫体内的抗冻保护物质和生理指标并进行分析。【结果】1)越冬幼虫主要为6龄幼虫和少量5龄幼虫。2)5龄幼虫过冷却点为(-19.25±0.66)℃,体液结冰点为(-12.44±0.60)℃。6龄幼虫过冷却点为(-14.32±0.91)℃,体液结冰点为(-8.00±0.72)℃。3)同虫龄不同个体过冷却点和体液结冰点变异均符合正态分布。4)体长在[6.00,9.00)mm的赤松梢斑螟幼虫过冷却点为(-19.26±0.57)℃,体液结冰点为(-12.07±0.47)℃;体长在[12.00,15.00)mm的赤松梢斑螟幼虫过冷却点为(-12.18±1.35)℃,体液结冰点为(-6.22±0.90)℃。质量在[10.00,30.00)mg的赤松梢斑螟幼虫过冷却点为(-18.91±0.69)℃,体液结冰点为(-11.57±0.33)℃;质量在[70.00,90.00)mg的赤松梢斑螟幼虫过冷却点为(-13.09±1.90)℃,体液结冰点为(-6.43±1.22)℃。5)幼虫体长从越冬期的(10.25±0.22)mm增加至越冬后期的(14.42±0.22)mm;质量从(53.90±2.80)mg增至(63.20±2.30)mg;含水率从(51.14±0.02)%增至(59.58±0.02)%;甘油三酯含量从越冬期的(47.74±29.94)%降低至越冬后期的(34.18±30.19)%;总蛋白含量从越冬期的(230.06±14.82)mg/g降低至越冬后期的(73.33±13.03)mg/g;甘油含量从越冬期的(1889.23±116.93)μg/g增加至越冬后期的(6655.54±223.85)μg/g;过氧化物酶(POD)活性从越冬期的(68.79±10.40)U/g降低至越冬后期的(17.32±3.24)U/g;过氧化氢酶(CAT)活性从越冬期的(161024.33±7574.07)U/g降低至越冬后期的(134039.69±4308.14)U/g;超氧化物歧�【Objective】The cold resistance of overwintering larvae of Dioryctria sylvestrella and the physiological and biochemical indexes of different overwintering stages were determined to provide basis for the study of overwintering behavior of D.sylvestrella,and their potential geographical distribution in China.【Method】The overwintering larval instars of D.sylvestrella were determined by the method of tree dissection and inspection in the overwintering period.The supercooling point and freezing point of the overwintering larvae of D.sylvestrella were measured by using the supercooling point tester,and the relationship between the supercooling point and the larval instar,body length and body weight were analyzed.The antifreeze protective substances and physiological indexes of larvae in overwintering period and late overwintering period were determined and analyzed.【Result】1)The overwintering larvae were mainly 6th instar larvae and a small number of 5th instar larvae;2)The supercooling point of the 5th instar larvae was(-19.25±0.66)℃,and the freezing point was(-12.44±0.60)℃.The supercooling point of the 6th instar larvae was(-14.32±0.91)℃,and the freezing point was(-8.00±0.72)℃;3)The variation of supercooling point and freezing point of different individuals of the same instar conformed to normal distribution;4)The supercooling point(SCP)and freezing point(FP)of larvae with body length of[6.00,9.00)mm were(-19.26±0.57)℃and(-12.07±0.47)℃.The supercooling point of the larvae with body length of[12.00,15.00)mm was(-12.18±1.35)℃,and the freezing point was(-6.22±0.90)°C.The supercooling point and freezing point of the larvae with body weight of[10.00,30.00)mg were(-18.91±0.69)℃and(-11.57±0.33)℃.The supercooling point and freezing point of the larvae with body weight of[70.00,90.00)mg were(-13.09±1.90)℃and(-6.43±1.22)℃;5)The larval body length increased from(10.25±0.22)mm in the overwintering period to(14.42±0.22)mm in the late overwintering period.The body weight increased f

关 键 词：赤松梢斑螟 过冷却点 体液结冰点 耐寒性 耐寒物质 

分 类 号：S763.301[农业科学—森林保护学]