盐碱胁迫下不同抗性杨树品种转录组分析  

Transcriptome analysis of different resistant poplar varieties under salt alkali stress

作　　者：李文颖 李晓宇 杨成超 LI Wenying;LI Xiaoyu;YANG Chengchao(Poplar Research Institute of Liaoning Province,Gaizhou 115200,Liaoning,China)

机构地区：[1]辽宁省杨树研究所,辽宁盖州115200

出　　处：《中南林业科技大学学报》2025年第2期122-130,共9页Journal of Central South University of Forestry & Technology

基　　金：国家科技创新2030-重大项目(2023ZD0405603);辽宁省农业科学院院长基金项目(2023QN2416)。

摘　　要：【目的】研究不同抗性杨树品种响应盐碱胁迫的转录水平,挖掘杨树耐盐碱相关基因,为后续深入探讨杨树响应盐碱胁迫的分子机制及后续遗传转化提供理论支撑。【方法】以杨树耐盐碱品种‘辽胡1号’和盐碱敏感品种‘中辽1号’为材料,采用盆栽法,加入NaHCO_(3)溶液模拟盐碱胁迫,利用转录组测序技术、生物信息学等方法分析盐碱胁迫下不同抗性杨树品种的转录水平,筛选与盐碱胁迫相关的差异表达基因,随机选取6个差异表达基因进行实时荧光定量PCR试验,验证转录组数据的准确性。【结果】转录组测序结果表明,盐碱胁迫下不同抗性杨树品种之间共获得5273个差异表达基因,其中上调表达2709个,下调表达2564个。GO分析结果发现,共有4203个差异基因被注释到3大类37条通路中。KEGG分析结果发现,共有3616个差异基因被注释,差异基因显著富集在植物信号转导途径(植物与病原体相互作用、植物激素信号转导)、代谢途径(淀粉与蔗糖代谢、碳代谢)和生物合成途径(苯丙素的生物合成、氨基酸的生物合成)。盐碱胁迫下MYB、ABC、WRKY、bHLH是差异基因较多的基因家族,筛选出CBL4、SOD2、bHLH35、ABCC10、CYP736A12、P5CS等差异基因表达显著上调。qRT-PCR结果验证转录组数据结果可靠。【结论】本研究通过转录组测序技术对盐碱胁迫下不同抗性杨树品种进行比较分析,挖掘出与耐盐碱性相关差异表达基因,为后续基因克隆和遗传转化奠定理论基础。【Objective】To study the transcriptional levels of different resistant poplar varieties in response to salt alkali stress,explore the genes related to salt alkali tolerance in poplar,and provide theoretical support for further exploration of the molecular mechanisms of poplar response to salt alkali stress and subsequent genetic transformation.【Method】Using poplar salt alkali tolerant variety P.simonii×P.euphratica and salt alkali sensitive variety P.×canadensiscv.‘Zhongliao1’as materials,using the potted plant method,NaHCO_(3) was added to simulate salt alkali stress.Transcriptome sequencing technology,bioinformatics,and other techniques were used to analyze the transcription levels of different resistant poplar varieties under salt alkali stress.Differential expression genes related to salt alkali stress were screened,and six differentially expressed genes were randomly selected for real-time fluorescence quantitative PCR testing to verify the accuracy of transcriptome data.【Result】Through transcriptome sequencing,it was found that under salt alkali stress,different resistant poplar varieties obtained a total of 5273 differentially expressed genes,of which 2709 were upregulated and 2564 were downregulated.GO analysis results revealed that a total of 4203 differentially expressed genes were annotated into 37 pathways across three major categories.The KEGG analysis results showed that a total of 3616 differentially expressed genes were annotated,and the differentially expressed genes were significantly enriched in plant signal transduction pathways(plant pathogen interaction,plant hormone signal transduction),metabolic pathways(starch and sucrose metabolism,carbon metabolism),and biosynthetic pathways(phenylpropanoid biosynthesis,amino acid biosynthesis).MYB,ABC,WRKY,and bHLH are gene families with more differentially expressed genes under salt alkali stress.CBL4,SOD2,bHLH35,ABCC10,CYP736A12,P5CS and other differentially expressed genes were significantly upregulated through screening.The qRT PCR

关 键 词：杨树 盐碱胁迫 转录组测序 差异基因 

分 类 号：S792.11[农业科学—林木遗传育种]