基于慢性炎性疼痛模型的艾灸抗炎镇痛介入时机研究  

Optimal Timing of Moxibustion Intervention for Anti-inflammatory and Analgesic Effects Based on a Chronic Inflammatory Pain Model

作　　者：周蕊竹 何柳萱 侯帅 杨莎[1,2,3] 尹海燕 余曙光[1,2,3] ZHOU Ruizhu;HE Liuxuan;Hou Shuai;YANG Sha;YIN Haiyan;YUShuguang(Acupuncture and Tuina School,Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China;Key Laboratory of Acupuncture for Senile Disease(Chengdu University of TCM),Ministry of Education,Chengdu 610075,China;Acupuncture and Chronobiology Key Laboratory of Sichuan Province,Chengdu 610075,China)

机构地区：[1]成都中医药大学针灸推拿学院,成都610075 [2]针灸防治老年疾病教育部重点实验室,成都610075 [3]针灸与时间生物学四川省重点实验室,成都610075

出　　处：《四川大学学报(医学版)》2025年第1期137-142,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(No.82230127、No.81973969)资助。

摘　　要：目的基于完全弗氏佐剂(complete Freund's adjuvant,CFA)诱导的慢性炎性疼痛模型,对比分析不同艾灸介入时间的抗炎镇痛效应差异,探寻艾灸抗炎镇痛的适宜介入时机,为艾灸抗炎镇痛的基础研究探寻规范的干预方案。方法实验分为三部分,分别在造模后第4、7、10天开展艾灸介入时间的研究。每部分研究将雄性C57BL/6小鼠随机分为空白组、模型组和艾灸介入组,每组各8只。通过小鼠右侧足底注射20μL完全弗氏佐剂建立慢性炎性疼痛模型。分别于造模后第4、7、10天给予“足三里”穴艾灸干预30 min,连续干预7天。于造模前和造模后以及艾灸治疗第1、4、7天检测热辐射刺激缩爪潜伏期评价痛阈,检测足容积评价足趾肿胀度。结果与空白组比,模型组痛阈均降低(P<0.0001),足容积均增高(P<0.0001)。与模型组比,造模后第4、7、10天艾灸介入组痛阈升高(P<0.05,P<0.0001);第4天艾灸介入组足容积反而升高(P<0.0001),第7、10天艾灸介入组足容积降低(P<0.0001)。第4、7、10天各艾灸介入组组间比较,第7天艾灸介入组痛阈升高显著(P<0.05,P<0.0001),第7、10天艾灸介入组足容积降低显著(P<0.0001)。结论综合艾灸镇痛和抗炎两方面效应来看,造模后第7天可能是艾灸发挥抗炎镇痛效应的适宜介入时机。Objective Based on a complete Freund's adjuvant(CFA)-induced chronic inflammatory pain model,we compared and analyzed the differences in anti-inflammatory and analgesic effects of moxibustion intervention initiated at different timepoints,aiming to identify the optimal timing for moxibustion intervention.The goal is to establish standardized intervention protocols for basic research on the anti-inflammatory and analgesic effects of moxibustion.Methods Male C57BL/6 mice were randomly divided into 3 groups based on the moxibustion initiation timepoints of 4,7,and 10 d after modeling.Then,the mice in each group were randomly assigned to 3 subgroups,including a control group,a model group,and a moxibustion group,with 8 mice in each subgroup.Chronic inflammatory pain was induced by injecting 20μL of CFA into the sole of the right hind paw.Moxibustion applied at the"Zusanli"acupoint for 30 minutes started on the 4th,7th,and 10th days after modeling,and the intervention continued for 7 days.The latency of paw withdrawal to thermal radiation was measured to evaluate the pain threshold before modeling,after modeling,and on the 1st,4th,and 7th days of treatment.Foot volume was measured to assess toe swelling before modeling,after modeling,and on the 1st and 7th days of treatment.Results Compared with the control group,the model group exhibited a reduced pain threshold(P<0.0001)and increased paw volume(P<0.0001).Compared with the model group,the subgroups receiving moxibustion intervention initiated on the 4th,7th,and 10th days post-modeling exhibited an increased pain threshold(P<0.05,P<0.0001).However,the paw volume of the subgroups receiving moxibustion intervention initiated on the 4th day post-modeling increased(P<0.0001),while those of the subgroups receiving moxibustion intervention initiated on the 7th and 10th days post-modeling decreased(P<0.0001).Among the intervention subgroups receiving moxibustion initiated on days 4,7,and 10,the day 7 intervention-initiating subgroup showed significant increase in pain thres

关 键 词：艾灸 介入时机 慢性炎性疼痛 

分 类 号：R245.81[医药卫生—针灸推拿学]