普鲁士蓝纳米颗粒延缓衰老性肌萎缩的作用研究  

Effect of Prussian blue nanoparticles on delaying aging muscle atrophy

作　　者：张树宝 杨进 常圣杰 徐浩伟 易宇阳 李恺[3] 王善金 ZHANG Shu-bao;YANG Jin;CHANG Sheng-jie;XU Hao-wei;YI Yu-yang;LI Kai;WANG Shan-jin(Department of Spinal Surgery,Shanghai East Hospital,School of Medicine,Tongji University,Shanghai 200092,China;Department of Orthopedics,Ji an Central People’s Hospital,Ji an 343008,Jiangxi,China;Shanghai Institute of Silicate,Chinese Academy of Sciences,Shanghai 201899,China)

机构地区：[1]同济大学医学院同济大学附属东方医院脊柱外科,上海200092 [2]吉安市中心人民医院骨科,江西吉安343008 [3]中国科学院上海硅酸盐研究所,上海201899

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2025年第1期73-82,共10页Chinese Journal Of Osteoporosis And Bone Mineral Research

基　　金：上海市浦东新区卫生系统学科建设特色专病项目(PWZZb2022-22);江西省吉安市科技计划项目(20222-201787);上海市浦东新区卫生健康委员会学科带头人培养计划(PWRd2022-11)。

摘　　要：目的验证普鲁士蓝纳米颗粒(Prussian blue nanoparticles,PBNPs)体内外调控C2C12细胞抗氧化和改善线粒体功能的能力及改善衰老性肌萎缩的效能。方法制备PBNPs并进行表征。体外实验:检测PBNPs分解H_(2)O_(2)和·O_(2)^(-)的能力和生物相容性;在H_(2)O_(2)条件下,检测PBNPs对C2C12细胞的保护作用、活性氧荧光表达及线粒体功能。体内实验:小鼠腹腔注射D-半乳糖(D-galactose,D-gal)建立衰老性肌萎缩的模型,分对照组(未予治疗)、D-gal组及D-gal+PBNPs干预组,观察体质量和组织形态学变化。结果体外实验:PBNPs在25~100μg/mL质量浓度下对细胞无毒性;PBNPs对H_(2)O_(2)有降解活性且有很强的清除超氧阴离子自由基的能力;线粒体膜电位提示PBNPs可有效改善氧化应激条件下膜电位的下降及维持细胞内的三磷酸腺苷(adenosine triphosphate,ATP)含量。Western blot实验结果提示PBNPs干预后可显著提高C2C12细胞肌肉分化标记物(MyoD和MyoG)的蛋白表达水平。体内实验:4周后,D-gal组小鼠体质量显著下降,PBNPs干预后可显著逆转。小麦胚芽凝集素(wheat germ agglutinin,WGA)和Masson染色显示,与D-gal组比较,PBNPs干预组骨骼肌横截面积显著增加及骨骼肌组织纤维化显著改善。结论PBNPs可通过发挥抗氧化应激和改善线粒体功能的作用延缓衰老性肌萎缩的进程。Objective To evaluate the ability of Prussian blue nanoparticles(PBNPs)to regulate antioxidant defenses and improve mitochondrial function in C2C12 cells both in vitro and in vivo,and to assess their efficacy in mitigating aging-related muscle atrophy.Methods PBNPs were synthesized and characterized.The capacity of PBNPs to degrade H_(2)O_(2) and superoxide anions(·O_(2)^(-))was assessed,along with their biocompatibility in in vitro experiments.Under H_(2)O_(2)-induced oxidative stress conditions,the protective effects of PBNPs on C2C12 cells,reactive oxygen species(ROS)fluorescence expression and mitochondrial function were evaluated.An aging-related muscle atrophy model was established via intraperitoneal injection of D-galactose(D-gal)in mice for in vivo experiments.Mice were divided into control(untreated),D-gal,and D-gal+PBNPs intervention groups.Changes in body weight and tissue histomorphology were observed.Results From in vitro experiments,PBNPs exhibited no cytotoxicity at concentrations ranging from 25 to 100μg/mL.PBNPs demonstrated significant H_(2)O_(2) degradation activity and robust superoxide anion radical scavenging ability.Mitochondrial membrane potential assessments indicated that PBNPs effectively prevented the decline in membrane potential under oxidative stress and maintained intracellular adenosine triphosphate(ATP)levels.Western blot analysis showed that PBNPs significantly upregulated the protein expression levels of muscle differentiation markers MyoD and MyoG in C2C12 cells following intervention.After 4 weeks,the D-gal group of mice showed a significant decrease in body weight,which was markedly reversed by PBNPs intervention.Wheat germ agglutinin(WGA)and Masson s trichrome staining revealed that compared to the D-gal group,the PBNPs intervention group exhibited a significant increase in skeletal muscle cross-sectional area and a notable reduction in muscle tissue fibrosis.Conclusion PBNPs can delay the progression of aging-related muscle atrophy by exerting antioxidant effects and im

关 键 词：普鲁士蓝纳米颗粒 衰老性肌萎缩 氧化应激 线粒体功能调控 

分 类 号：R69[医药卫生—泌尿科学]