Akkermansia muciniphila isolated from forest musk deer ameliorates diarrhea in mice via modification of gut microbiota  

作　　者：Yan Deng Yan Wang Ying Liu Xiaoli Yang Hai Zhang Xiaochang Xue Yi Wan 

机构地区：[1]Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry,College of Life Sciences,Shaanxi Normal University,Xi'an,China [2]Shaanxi Institute of Microbiology,Xi'an,China [3]NMPA Key Laboratory for Testing Technology of Pharmaceutical Microbiology,Shaanxi Institute for Food and Drug Control,Xi'an,China [4]Department of Cell Biology,National Translational Science Center for Molecular Medicine,Fourth Military Medical University,Xi'an,China

出　　处：《Animal Models and Experimental Medicine》2025年第2期295-306,共12页动物模型与实验医学(英文)

基　　金：This research was funded by Shaanxi Administration of Traditional Chinese Medicine Projects(2021-QYZL-01,2021-QYPT-001);Key R&D Project in Shaanxi Province(2023-YBSF-463);the Foundation of Science and Technology in Shaanxi Province(2020TD-050);the Fundamental Research Funds for the Central Universities(GK202205010).

摘　　要：Background:The forest musk deer,a rare fauna species found in China,is famous for its musk secretion which is used in selected Traditional Chinese medicines.However,over-hunting has led to musk deer becoming an endangered species,and their survival is also greatly challenged by various high incidence and high mortality respiratory and intestinal diseases such as septic pneumonia and enteritis.Accumulating evidence has demonstrated that Akkermannia muciniphila(AKK)is a promising probiotic,and we wondered whether AKK could be used as a food additive in animal breeding pro-grammes to help prevent intestinal diseases.Methods:We isolated one AKK strain from musk deer feces(AKK-D)using an im-proved enrichment medium combined with real-time PCR.After confirmation by 16S rRNA gene sequencing,a series of in vitro tests was conducted to evaluate the probiotic effects of AKK-D by assessing its reproductive capability,simulated gas-trointestinal fluid tolerance,acid and bile salt resistance,self-aggregation ability,hy-drophobicity,antibiotic sensitivity,hemolysis,harmful metabolite production,biofilm formation ability,and bacterial adhesion to gastrointestinal mucosa.Results:The AKK-D strain has a probiotic function similar to that of the standard strain in humans(AKK-H).An in vivo study found that AKK-D significantly amelio-rated symptoms in the enterotoxigenic Escherichia coli(ETEC)-induced murine diar-rhea model.AKK-D improved organ damage,inhibited inflammatory responses,and improved intestinal barrier permeability.Additionally,AKK-D promoted the reconsti-tution and maintenance of the homeostasis of gut microflora,as indicated by the fact that AKK-D-treated mice showed a decrease in Bacteroidetes and an increase in the proportion of other beneficial bacteria like Muribaculaceae,Muribaculum,and unclas-sified f_Lachnospiaceae compared with the diarrhea model mice.Conclusion:Taken together,our data show that this novel AKK-D strain might be a potential probiotic for use in musk deer breeding,although further extensive syste

关 键 词：Akkermansia muciniphila DIARRHEA enterotoxigenic Escherichia coli forest musk deer gut microbiota 

分 类 号：S816.7[农业科学—饲料科学]