兔大型艾美耳球虫四川中江株的分离与鉴定  

Isolation and identification of the SCzj strain of Eimeria Magna in Rabbits

作　　者：朱煜华 许力文 汤春佳 马义博 任永军[2] 杨光友[1] ZHU Yuhua;XU Liwen;TANG Chunjia;MA Yibo;REN Yongjun;YANG Guangyou(College of Veterinary Medicine,Sichuan Agricultural University,Chengdu 611130;Sichuan Animal Science Academy,Chengdu 610066)

机构地区：[1]四川农业大学动物医学院,成都611130 [2]四川省畜牧科学研究院,成都610066

出　　处：《中国养兔》2024年第6期4-8,共5页Chinese Journal of Rabbit Farming

摘　　要：分离鉴定兔大型艾美耳球虫,为兔大型艾美耳球虫的防控及相关研究奠定基础。通过单卵囊分离技术,从病兔粪样中分离疑似大型艾美耳球虫虫株,通过形态学观察和生物学特性进行初步鉴定,随后进一步应用内转录间隔区1(ITS-1)序列测定与分析对虫种进行分子鉴定。根据对该分离虫株的形态学和生物学特性观察初步鉴定为兔大型艾美耳球虫,该分离株ITS-1序列与GenBank中发布的兔大型艾美耳球虫甘肃株(OP376059.1)和河南株(MT982785.1)序列相似性达100%。该分离虫株鉴定为兔大型艾美耳球虫四川中江株(Eimeria magna SCzj)。Isolation and identification of Eimeria magna in rabbits,laying the foundation for the prevention and control and related research of Eimeria magna.The suspected Eimeria magna strains were isolated from faecal samples of infected rabbits by the technique of single oocyst isolation,The strain was preliminarily identified through morphological observation and biological characteristics,and then further molecular identification of the species was carried out by sequencing and analyzing the internal transcribed spacer 1(ITS-1)region.Based on the observation of the morphological and biological characteristics of the isolated strain,it was preliminarily identified as Eimeria magna of rabbits.The ITS-1 sequence of this isolate had a 100%sequence similarity with the Eimeria magna strains from Gansu(OP376059.1)and Henan(MT982785.1)published in GenBank.The isolated strain was identified as the SCzj strain of Eimeria magna in rabbits(Eimeria magna SCzj).

关 键 词：兔 大型艾美耳球虫 单卵囊分离 ITS-1序列 

分 类 号：S829.1[农业科学—畜牧学]