金霉素和多黏菌素配伍对家兔流行性腹泻病的防治效果研究  

Study on the Effect of Chlortetracycline and Polymyxin on Epidemic Diarrhea in Rabbits

作　　者：刘世洋 肖浩然 张自强[1,2] 李帅鹏 张智凯 刘玉梅 LIU Shiyang;XIAO Haoran;ZHANG Ziqiang;LI Shuaipeng;ZHANG Zhikai;LIU Yumei(College of Animal Science and Technology,Henan University of Science and Technology,Luoyang 471000;Rabbit Research and Development Center of Henan Province,Luoyang 471000;Jinhe Biotechnology Company Limited,Hohhot 010000)

机构地区：[1]河南科技大学动物科技学院,洛阳471000 [2]河南省兔业研发中心,洛阳471000 [3]金河生物科技股份有限公司,呼和浩特010000

出　　处：《中国养兔》2024年第6期15-18,30,共5页Chinese Journal of Rabbit Farming

基　　金：校企横向合作项目资助项目(横20230158、横20240019)。

摘　　要：旨在探究金霉素和多黏菌素联合应用对家兔流行性腹泻病的防治效果。选取40只35日龄健康家兔随机分为两组,每组20只,分别为感染组和治疗组。试验开始前,通过最低抑菌浓度(MIC)法测定大肠埃希菌和魏氏梭菌对金霉素和多黏菌素的最小抑菌浓度。试验第1天,每只家兔按照1:1的比例灌胃2 mL含有1×10^(9) cfu/mL的大肠埃希菌和魏氏梭菌的菌液;试验第2天,治疗组家兔每天分别灌胃100 mg/kg BW的金霉素和多黏菌素至试验结束,试验期共7 d。试验结束后,采集家兔空肠和胃组织通过H.E染色观察组织形态学变化。MIC试验结果显示,金霉素和多黏菌素联合使用时对大肠埃希菌和魏氏梭菌抑菌效果均明显。H.E染色结果显示,感染组家兔空肠空肠绒毛排列紊乱;肠绒毛部分溶解脱落;固有层中心附近出现红细胞和炎性细胞浸润;黏膜上皮细胞数量增多。胃壁结构受损,胃黏膜上皮细胞坏死脱落;黏膜下层出现大量炎性细胞。与感染组相比,经过金霉素和多黏菌素治疗后,家兔空肠和胃组织的病理损伤明显改善。说明金霉素和多黏菌素配伍对大肠埃希菌和魏氏梭菌有明显的抑菌效果,对家兔流行性腹泻病有良好治疗作用。This study aims to explore the preventive and therapeutic effects of the combination of streptomycin and polymyxin on epidemic diarrhea in rabbits.Forty 35 day old healthy rabbits were randomly divided into two groups,with 20 rabbits in each group,namely the infection group and the treatment group.Before the experiment,the minimum inhibitory concentrations of Escherichia coli and Clostridium welchii against streptomycin and polymyxin were determined by MIC method.On the first day of the experiment,each rabbit was orally administered 2 mL of bacterial solution containing 1×10^(9)cfu/mL of Escherichia coli and Clostridium welchii in a 1:1 ratio;On the second day of the experiment,rabbits in the treatment group were orally administered 100 mg/kg BW of streptomycin and polymyxin each day until the end of the experiment,for a total of 7 d.After the experiment,rabbit jejunum and stomach tissues were collected and observed for histological changes through HE staining.The MIC test results showed that the combination of streptomycin and polymyxin had significant antibacterial effects on Escherichia coli and Clostridium welchii.H.The E staining results showed that the villi arrangement of the jejunum in the infected group of rabbits was disordered;Partial dissolution and shedding of intestinal villi;There is infiltration of red blood cells and inflammatory cells near the center of the lamina propria;The number of mucosal epithelial cells increases.Damage to the gastric wall structure,necrosis and shedding of gastric mucosal epithelial cells;A large number of inflammatory cells appear in the submucosal layer.Compared with the infected group,after treatment with streptomycin and polymyxin,the pathological damage of the rabbit's jejunum and stomach tissues was significantly improved.The combination of streptomycin and polymyxin has a significant antibacterial effect on Escherichia coli and Clostridium welchii,and has a good therapeutic effect on epidemic diarrhea in rabbits.

关 键 词：金霉素 多黏菌素 家兔 大肠埃希菌 魏氏梭菌 

分 类 号：S829.1[农业科学—畜牧学]