基质金属蛋白酶9基因rs3918242多态性与2型糖尿病患者合并单纯性肾囊肿风险相关性的研究  

Association of rs3918242 polymorphism in MMP-9 gene with the risk of renal cyst in patients with type 2 diabetes mellitus

作　　者：贺超贵 李可 尹述恒 雷景春 刘凡 陈克勤 龚道星 杨博 李波 HE Chaogui;LI Ke;YIN Shuheng(Department of Vascular Surgery,Changsha First Hospital,Changsha 410000,China)

机构地区：[1]长沙市第一医院血管外科,410000 [2]长沙市第一医院内分泌科,410000

出　　处：《中国糖尿病杂志》2025年第1期43-48,共6页Chinese Journal of Diabetes

基　　金：长沙市卫健委2023年度科研立项课题(KJ-B2023061)。

摘　　要：目的探讨基质金属蛋白酶9(MMP-9)基因rs3918242多态性与T2DM患者合并单纯性肾囊肿(SRCs)风险的相关性。方法选取2020年6月至2023年12月本院内分泌科收治的门诊及住院T2DM患者483例,根据是否合并SRCs分为单纯T2DM组(n=355)和合并SRCs组(SRCs,n=128)。比较两组一般资料及生化指标,Spearman相关分析rs3918242多态性与T2DM合并SRCs的相关性,LASSO回归和逐步线性回归分析T2DM合并SRCs的影响因素,Logistic多因素回归分析T2DM合并SRCs的独立危险因素。结果SRCs组MMP-9高于T2DM组(P<0.05)。SRCs组MMP-9基因rs3918242基因型CT/TT与等位基因T分布频率高于T2DM组(P<0.05)。Spearman相关分析显示,MMP-9基因rs3918242基因型CT/TT与T2DM合并SRCs呈正相关(r=0.344、0.150,P<0.05),等位基因T频率与T2DM合并SRCs呈正相关(r=0.355,P=0.000)。多元线性逐步回归分析显示,年龄、FPG、血肌酐(Scr)、白蛋白、血小板计数、e GFR、血钙、MMP-9及rs3918242基因型CT/TT是T2DM合并SRCs的影响因素。Logistic回归分析显示,FPG、白蛋白、e GFR、Scr、MMP-9及rs3918242基因型CT/TT是T2DM合并SRCs的影响因素。结论T2DM合并SRCs患者血清MMP-9水平升高,且rs3918242基因型CT/TT是T2DM患者合并SRCs的危险因素。Objective To investigate the association between the rs3918242 polymorphism in the matrix metalloproteinase-9(MMP-9)gene and the risk of simplerenal cysts(SRCs) in patients with type 2diabetes mellitus(T2DM). Methods A total of 483 outpatient with T2DM and inpatient with T2DM admitted to endocrinology department in our hospital were enrolledin this study from June 2020 to December 2023. All the patients were divided into two groups according to the diagnostic criteria for SRCs:T2DM group(n=355) and T2DM with SRCs group(SRCs,n=128). General clinical characteristics and biochemical indicators were compared between the two groups. The correlation between rs3918242polymorphism and T2DM with SRCs was analyzed by spearman correlation. LASSO regression and stepwise linear regression were employed to identify factors influencing T2DM with SRCs. Multivariate logistic regression analysis was performed to determine the independent risk factors for SRCsin patients with T2DM. Results Serum MMP-9 level was higher in SRCs group than in T2DM group(P<0. 05).The frequency of the rs3918242 CT/TT genotypes and T allele was significantly higher in SRCs group than in T2DM group(P<0. 05). Spearman correlation analysis demonstrateda positive correlation betweenthers3918242 genotype CT/TT and the presence of SRCs(r=0. 344,0. 150,P<0. 05) and between the T allele frequency and SRCs(r=0. 355,P=0. 000) in patients with T2DM. Stepwise linear regression identified age,fasting plasma glucose,serum creatinine,albumin,platelet count,estimated glomerular filtration rate(e GFR),calcium levels,MMP-9,and rs3918242 CT/TT genotype as influencing factors for SRCs in T2DM patients. Logistic regression analysis revealed that FPG,albumin,e GFR,Scr,MMP-9,and the rs3918242 CT/TT genotype were independent risk factors for SRCs in patients with T2DM.Conclusions Serum MMP-9 levels areelevatedin T2DM patients with SRCs,and rs3918242 CT/TT genotype in MMP-9 gene areat increased risk for developing SRCs.

关 键 词：糖尿病 2型 单纯性肾囊肿 基质金属蛋白酶9基因多态性 危险因素 

分 类 号：R587.2[医药卫生—内分泌] R692[医药卫生—内科学]