MCC950对感染性早产大鼠子宫平滑肌收缩的改善作用及可能机制  

Ameliorating effect of MCC950 on uterine smooth muscle contraction in infected preterm rats and its possible mechanism

作　　者：张金环 安娜 孙琳 夏艳秋 孙静莉 ZHANG Jin-huan;AN Na;SUN Lin;XIA Yan-qiu;SUN Jing-li(Department of Obstetrics and Gynecology Northern Theater Command General Hospital,Shenyang 110013;Department of Radiotherapy,Northern Theater Command General Hospital,Shenyang 110013;Laozhan Stret Community Health Service Center,Dongchang District,Tonghua 134001;Department of Pediatrics,Northern Theater Command General Hospital,Shenyang 110013,China)

机构地区：[1]北部战区总医院妇产科,辽宁沈阳110013 [2]北部战区总医院放疗科,辽宁沈阳110013 [3]通化市东昌区老站街道社区卫生服务中心,吉林通化134001 [4]北部战区总医院儿科,辽宁沈阳110013

出　　处：《解剖科学进展》2024年第6期617-620,共4页Progress of Anatomical Sciences

基　　金：辽宁省自然科学基金(2019-ZD-1059)。

摘　　要：目的研究NLRP3抑制剂MCC950对LPS诱导产生的感染性早产大鼠子宫平滑肌收缩的改善作用及可能机制。方法将怀孕SPF级Wistar大鼠分为对照组(Control组),LPS诱导的感染性早产组(LPS组)和NLRP3抑制剂组(NLRP3组)。在孕15 d时,采血后安乐死孕鼠,取子宫平滑肌组织,检测离体子宫肌张力变化,HE染色观察子宫组织病理变化,Elisa检测血清中IL-1β和IL-18的表达,Western blot检测子宫平滑肌钙离子通道蛋白Cav1.2、Cav3.1及Cav3.2,焦亡相关蛋白GSDMD-N、GSDMD-G、Pro-Caspase-1蛋白的表达,免疫荧光检测ASC、NLRP3的表达。结果NLRP3抑制剂MCC950明显改善LPS刺激引起的感染性早产子宫组织炎性浸润,降低子宫肌张力,降低血清中IL-1β、IL-18的水平,下调钙离子通道蛋白Cav1.2、Cav3.1及Cav3.2的表达;抑制ASC、NLRP3、GSDMD-N、GSDMD-G的表达,上调Pro-Caspase-1的表达。结论NLRP3抑制剂能改善感染性早产大鼠子宫平滑肌收缩功能,抑制钙离子内流,抑制子宫平滑肌细胞焦亡。Objective To study the effect of NLRP3 inhibitor MCC950 on uterine smooth muscle contraction induced by LPS-induced infectious preterm birth rats and its possible mechanism.Methods Pregnant SPF Wistar rats were divided into control group,LPS-induced infectious preterm birth group and NLRP3 inhibitor group.On the 15d of gestation,the pregnant mice were euthanized after blood collection,and the uterine smooth muscle tissues were collected to detect the changes of uterine muscle tone in vitro.HE staining was used to observe the pathological changes of uterine tissue.ELISA was used to observe the levels of IL-1βand IL-18 in serum.Western blot was used to detect the expressions of calcium channel proteins Cav1.2,Cav3.1,Cav3.2 and the pyrogen related proteins GSDMD-N,GSDMD-G and Pro-Caspase-1 in uterine smooth muscle.The expressions of ASC and NLRP3 were detected by immunofluorescence.Results NLRP3 inhibitor MCC950 significantly improved the inflammatory infiltration of infectious preterm birth uterus tissue induced by LPS stimulation,decreased the uterine muscle strip tension,decreased the levels of IL-1β and IL-18 in serum,and down-regulated the expressions of calcium channel proteins Cav1.2,Cav3.1 and Cav3.2.The expression of ASC,NLRP3,GSDMD-N,GSDMD-G was inhibited,and the expression of Pro-Caspase-1 was up-regulated.Conclusion NLRP3 inhibitor can protect uterine smooth muscle contraction,inhibit calcium ion flow and inhibit the scorch death of uterine smooth muscle cells in infected preterm rats.

关 键 词：NLRP3抑制剂 感染性早产 ASC/CASPASE1信号通路 焦亡 

分 类 号：R714.21[医药卫生—妇产科学]